冠状病毒Coronavirusl论文-2018 Co-localization of Middle East respiratory syndrome coronavirus (MERS-CoV) and dipeptidyl peptidase-4 in the respir.pdf

冠状病毒Coronavirusl论文-2018 Co-localization of Middle East respiratory syndrome coronavirus (MERS-CoV) and dipeptidyl peptidase-4 in the respir.pdf

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页数:29页

时间:2020-03-16

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1、DR.JOAQUIMSEGALÉS(OrcidID:0000-0002-1539-7261)Articletype:OriginalArticleCo-localizationofMiddleEastrespiratorysyndromecoronavirus(MERS-CoV)anddipeptidylpeptidase-4intherespiratorytractandlymphoidtissuesofpigsandllamasRunningtitle:MERS-CoV/DPP4co-localizationinpigsandllamasArticleAuthors

2、:NigeerTe,JúliaVergara-Alert,AnnikaLehmbecker,MónicaPérez,BartL.Haagmans,WolfgangBaumgärtner,AlbertBensaid,JoaquimSegalés*Authoraffiliations:IRTA,CentredeRecercaenSanitatAnimal(CReSA,IRTA-UAB),CampusdelaUniversitatAutònomadeBarcelona,08193Bellaterra,Spain(NigeerTe,J.Vergara-Alert,M.Pérez

3、,A.Bensaid);DepartmentofPathology,UniversityofVeterinaryMedicine,Hannover,Germany(A.Lehmbecker,W.Baumgärtner);DepartmentofViroscience,ErasmusMedicalCenter,3015CNRotterdam,TheNetherlands(B.L.Haagmans);UAB,CentredeRecercaenSanitatAnimal(CReSA,IRTA-UAB),CampusdelaUniversitatAutònomadeBarcel

4、ona,08193Bellaterra,SpainanddepartamentdeSanitatiAnatomiaAnimals,FacultatdeVeterinària,UAB,08193Bellaterra,Barcelona,Spain(J.Segalés).ThisarticlehasbeenacceptedforpublicationandundergonefullpeerreviewbuthasnotAcceptedbeenthroughthecopyediting,typesetting,paginationandproofreadingprocess,

5、whichmayleadtodifferencesbetweenthisversionandtheVersionofRecord.Pleasecitethisarticleasdoi:10.1111/tbed.13092Thisarticleisprotectedbycopyright.Allrightsreserved.*Correspondingauthor.Mailingaddress:CampusdelaUniversitatAutònomadeBarcelona,edificiCReSA,08193Bellaterra(CerdanyoladelVallès)

6、,Barcelona,Spain.Phone:(+34)934674040(Ext.1780);Fax:(+34)935814490.Emailaddress:joaquim.segales@irta.catAbstractThepresentstudyinvestigatedtheco-localizationoftheMiddleEastrespiratorysyndromecoronavirus(MERS-CoV)anditsreceptordipeptidylpeptidase-4(DPP4)acrossrespiratoryandlymphoidorganso

7、fexperimentallyMERS-CoVinfectedpigsandllamasbyArticleimmunohistochemistry(IHC).Also,scanningelectronmicroscopy(SEM)wasperformedtoassesstheciliaryintegrityofrespiratoryepithelialcellsinbothspecies.Inpigs,onday2post-inoculation(p.i.),DPP4-MERS-CoVco-localizationwasdetectedi

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