egf刺激肺癌细胞分泌il8并影响肺癌细胞生物学活性及其调控机制

《egf刺激肺癌细胞分泌il8并影响肺癌细胞生物学活性及其调控机制》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、优秀毕业论文复口_大学硕】学位论文录摘要第一部分摘要···································································································……1第二部分摘要···································································································一4前言································，····················

2、······························································一7第一部分PI3K/ERK通道和SPC一Al细胞分泌IL一8的关系·····································……9材料和方法··················································，····················································……10结果········································

3、·····································，······································……15讨论····················································································································……19第二部分PI3玲Akt和ERKI/2通道SPC一Al细胞的生物学特点的关系····················……21材料与方法······················

4、·················································································……22结果············································，····································，·，······，，························……30讨论············································、····································

5、··，································……37参考文献···········································································································……39致谢···················································································································……41附录l英文缩略词·······

6、··················································································……‘’42附录H综述···································································································……43附录IH已发表课题相关文章·······································································……55精品参考文献

7、资料优秀毕业论文复旦人学硕卜学位论文第一部分PI3K/ERK通道和SPC一Al细胞分泌IL一8的关系目的:探索EGF诱导NSCLC细胞SPC一Al分泌炎症因子IL一8及其可能的分子通道。方法:使用EGF(0.01ug/ml，0.1ug/ml和lug/ml)处理人NSCLC细胞24小时，ELIsA法测一上清中的ll.一8蛋白浓度。之后采用EGF(1oong/ml)单独和分别联合EGFR一Tki(厄洛替尼10卜M，l卜M)，PI3K抑市以剂GDC一0941不{JBEZ一235(l件M不!1IOOnM)，以及ERKI/2