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1、Castilloetal.,Transcriptomics2015,3:2mics:OptoeipnrAcscDOI:10.4172/2329-8936.1000120cranesTsTranscriptomics:OpenAccessISSN:2329-8936ResearchArticleOpenAccessGeneExpressionProfileandSignalingPathwaysinMCF-7BreastCancerCellsMediatedbyAcyl-CoASynthetase4OverexpressionAnaFCastill
2、o#,UlisesDOrlando#,PaulaLopez,AngelaRSolano,PaulaM.MalobertiandErnestoJPodesta*BiomedicalResearchInstitute,Inbiomed,DepartmentofBiochemistry,SchoolofMedicineUniversityofBuenosAires,CABA,C1121ABG,Argentina#equallyContributedAbstractAim:Breastcancercomprisesaheterogeneousgroupo
3、fdiseasesthatvaryinmorphology,biology,behaviorandresponsetotherapy.Previousstudieshaveidentifiedanacyl-CoAsynthetase4(ACSL4)gene-expressionpatterncorrelatedwithveryaggressivetumors.Inparticular,wehaveusedthetetracyclineTet-Offsystemtostablytransfectnon-aggressivebreastcancerM
4、CF-7cellsanddevelopedastablelineoverexpressingACSL4(MCF-7Tet-Off/ACSL4).Asaresult,wehaveproventhatcelltransfectionsolelywithACSL4cDNArendersahighlyaggressivephenotypeinvitroandresultsinthedevelopmentofgrowingtumorswheninjectedintonudemice.Nevertheless,andinspiteofwidespreadco
5、nsensusontheroleofACSL4inmediatinganaggressivephenotypeinbreastcancer,theearlystepsthroughwhichACSL4increasestumorgrowthandprogressionhavebeenscarcelydescribedandneedfurtherelucidation.Forthisreason,thegoalofthisworkwastostudythegeneexpressionprofileandthesignalingpathwaystri
6、ggeredbyACSL4overexpressioninthemechanismthatleadstoanaggressivephenotypeinbreastcancer.Methods:Wehaveperformedamassivein-depthmRNAsequencingapproachandareverse-phaseproteinarrayusingMCF-7Tet-Off/ACSL4cellsasamodeltoidentifygeneexpressionandfunctionalproteomicsignaturesspecif
7、ictoACSL4overexpression.ResultsandConclusion:ThesoleexpressionofACSL4displaysadistinctivetranscriptomeandfunctionalproteomicprofile.Furthermore,genenetworksmostsignificantlyupregulatedinbreastcancercellsoverexpressingACSL4areassociatedtotheregulationofembryonicandtissuedevelo
8、pment,cellularmovementandDNAreplicationandrepair.Inconclusion,ACSL4i
