A microfluidic model of the blood–brain barrier to study permeabilization by pulsed electric fields英文资料

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1、RSCAdvancesViewArticleOnlinePAPERViewJournal

2、ViewIssueAmicrofluidicmodeloftheblood–brainbarriertostudypermeabilizationbypulsedelectricfields†Citethis:RSCAdv.,2017,7,42811M.Bonakdar,*P.M.GraybillandR.V.DavalosPulsedelectricfieldsinteractwiththeblood–brainbarrier(

3、BBB)andhavebeenshowntoincreasetheBBBpermeabilityundersomepulsingregimes.PulsedelectricfieldsmayenhancedrugdeliverytothebrainbydisruptingtheintegrityoftheBBBandallowingotherwiseimpermeabledrugstoreachtargetareas.Microfluidic,invitromodelsofferanalternativeplatfor

4、mforexploringtheimpactofpulsedelectricfieldsontheBBBbecausetheycreatephysiologicallyrelevantmicroenvironmentsandeliminatetheconfoundingvariablesofanimalstudies.Wedevelopedamicrofluidicplatformforreal-timemeasurementofBBBpermeabilitypre-andpost-treatmentwithpuls

5、edelectricfields.Permeabilityismeasuredopticallybythediffusionoffluorescenttracersacrossamonolayerofhumancerebralmicrocapillaryendothelialcells(hCMECs)culturedonapermeablemembrane.WefoundthatthisdeviceReceived10thJuly2017isabletocapturereal-timepermeabilityofhCM

6、ECmonolayersforbothreversibleandirreversibleCreativeCommonsAttribution-NonCommercial3.0UnportedLicence.Accepted26thAugust2017electroporationpulsingregimes.Furthermore,preliminarytestingofdeepbrainstimulationpulsingDOI:10.1039/c7ra07603gregimesrevealspossiblei

7、mpactsonBBBintegrity.Thisdevicewillenablefuturestudiesofpulsedrsc.li/rsc-advanceselectricfieldregimesforimprovedunderstandingofBBBpermeabilization.Introductionbyhamperingdrugtransporttothebrain.TherearetwomajorpathwaysforthetransferofsubstancesacrosstheBBBwhic

8、harecategorizedasparacellularandtranscellular.5TransportCerebralmicrovesselsstrictlyregulatethetransferofsubstancesbetweenthebloodandthebraintissue.Thisregu-acrosstheintercellularTJs,theparacellularpathway,restrictsThisarticleislicensedunderalationbycerebralm

9、icro-vesselsiscalledtheblood–brainbarriertransporttosmallhydrophilicmoleculesoflessthan500Dainmolecularweight.5Transportthroughthecellmembraneand(BBB),andisduetointercellulartightjunction