自体表皮细胞-纤维蛋白膜创面移植治疗烧伤的实验分析

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1、天津医科大学硕士学位论文Abstractobjective1．Auto-epidermalcellstransplantingisaverygoodtherapeuticmethodincuringforsedouspatientswhoburnedinalargearea．Butintheprogressoftransplanting,theepidermalcellssuspensioncannotbelimitedinrawsurfaceofcutaneousdeficiencybecau

2、seofitsfluidity,SOthemaximalcuringeffectcannotbeplayed．Inordertosolvethisproblem，wewanttousefibrinmembraneasthematrixofepidermalcells．2．Tofindoutthebestdensityofinoculatingepidermalcellonfibrinmembrane，weinoculateepidermalcellsonfibrinmembraneandobse

3、rvetheirgrowth．Soweputtheepidermalcellsandthefibrinmembranetogether,andprovideafoundationforcuringburnedpatientswithcutaneousdeficiency．3．Inordertostudythefeasibilityofusingthefibrinmembraneofepidermalcellscurethebumrawsurfaceinlargearea．Inthisstudy,

4、withthehelpofourpreviousempiricalstudy,wewanttoputtheauto-epidermalcellsandfibrinmembranetogether,andtobuildthefibrinmembranewithepidermalcells，thenweputthisspecialmembraneontherat’Sbumrawsurfacealargearea．Methods1．Thethrombinandbloodplasmareneededin

5、thepreparationoffibrinmembrane．Weusedtheinjectedwatertodilutethrombinto1mh10IU，1mh50IUand1mh100IU．Thenwemadethreekindsofthefibrinmembraneusingthethrombinandbloodplasminequal-volume，andtome舔ureitsthicknessbythemicrometer．WeCangettheaveragevalue．Aboutm

6、easurethephysicsperformanceoffibrinmembrane，weusedthesamethicknesssamplewith1．5crnwidth，2．0tinlengthandeachl(inddofivesamplestomeasuretheindex．TocalculatebytheequationS=P／(A×B)andL=(L2一LI)／LI．Wetestthefibrinmembrane’Sbiocompatibilityanddegradationuse

7、themethodsofdirectobservationandhistology．2．Weusetrypsintodigesttheepidermis，andfinishtheisolationofrat’Sepidermalcells．Wecountcellsbythesliceofcellcounting,detectfigourofcellbytrypanbluedyeing．Weidentifythecellsbythewayofimmunehistochemistry．Weobser

8、vetheepidermalcellsmorpholo百cchangeandtheirgrowthstatusbylightmicroscope．Throughthecomparisonabouttherateofepidermalcellsadherence，wefindoutthesuitabledensityofinoculatingepidermalcells．Also，weputtheprimarycultureIII天津医科大学硕士学位论文epidermalcellsintothes