资源描述：
《AtGA2ox1 in a low input turfgrass Paspalum notatum Flugge reduces bioactive gibberellin》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、PlantBiotechnologyJournal(2007)5,pp.000–000doi:10.1111/j.1467-7652.2007.00284.xStableexpressionofPlantBiotechnologyJournalPBIBlackwellPublishingLtdOxford,UK1467-7644©2007TheAuthorsJournalcompilation©2007BlackwellPublishingLtdXXXOriginalArticlesExpressionofgibberellin2-oxidasei
2、mprovesturfqualityMRINALINIAGHARKARETAL.AtGA2ox1inalow-inputturfgrass(PaspalumnotatumFlugge)reducesbioactivegibberellinlevelsandimprovesturfqualityunderfieldconditions111,112MrinaliniAgharkar,PaulaLomba,FredyAltpeter*,HangningZhang,KevinKenworthyandTheoLange1AgronomyDepartment
3、,PlantMolecularBiologyProgram,GeneticsInstitute,InstituteofFoodandAgriculturalSciences,UniversityofFlorida,Gainesville,FL32611,USA2InstituteforPlantBiology,TechnicalUniversityofBrunswick,Mendelssohnstraße4,D-38106Braunschweig,GermanySummaryReceived7April2007;Bahiagrass(Paspalu
4、mnotatumFlugge)isaprimecandidateformolecularimprovementrevised6June2007;accepted12June2007.ofturfquality.Itspersistenceandlowinputcharacteristicsmadeitthedominantutility*Correspondence(fax3523927248;turfgrassalonghighwaysinthesouth-easternUSA.However,thecomparativelypoorturfe-
5、mailfaltpeter@ifas.ufl.edu)qualityduetoreducedturfdensityandprolificproductionofunsightlyinflorescencescurrentlylimitsthewidespreaduseofbahiagrassasresidentialturf.Alterationofendogenousgibberellin(GA)levelsbyapplicationofgrowthregulatorsortransgenicstrategieshasmodifiedplanta
6、rchitectureinseveralcrops.GAcatabolizingAtGA2ox1wassubclonedunderthecontroloftheconstitutivemaizeubiquitinpromoterandNos3’UTR.AminimalAtGA2ox1expressioncassettelackingvectorbackbonesequenceswasstablyintroducedintoapomicticbahiagrassbybiolisticgenetransferasconfirmedbySouthernb
7、lotanalysis.ExpressionofAtGA2ox1inbahiagrassasindicatedbyreversetranscription–polymerasechainreactionandNorthernblotanalysisresultedinasignificantreductionofendogenousbioactiveGA1levelscomparedtowildtype.Interestingly,transgenicplantsdisplayedanincreasednumberofvegetativetille
8、rswhichcorrelatedwiththelevelofAtGA2ox1expressionandenhancedt
