凝胶柱色谱(gel column choromatography)英文PPT

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1、ExperimentMethodsMasseyUniversityGelcolumnchromatographySeparationScienceContentDefinitionWhentouseMechanismAdvantages&disadvantagesAnexampleDefinitionofgelcolumnchromatographyGelcolumnchromatographyisakindofseparationmethodthatdependsontheprincipleofdiff

2、erentsizesofmoleculescanbeseparatedbytheporouspolymerbead,smallmoleculesenterthebeadwhilelargemoleculescan'tenterthebead,andmedium-sizemoleculespartiallyenterthebead.Whentouse?SeparationinChemistryAnalyticalChemistryPhytoChemistryNaturalMedicinalChemistry

3、BiochemistryOtherfieldsMechanismStationaryphase:Thephasethatisstationaryandcanseparateandpreservetheresearchsampleinchromatography.Mobilephase:Thesubstancesthatcarrytheanalytesandmoveforwardthroughthestationaryphaseinchromatography.Advantages&disadvantage

4、sAdvantages:1.Simple2.Goodseparationeffecttowardspolymericsubstances3.Maintainthebiologicalproperties4.EstimatedmolecularweightofthebiologicalmacromoleculesAdvantages&disadvantagesDisadvantages:1.Timeconsuming2.Needtobetakencareofcarefully3.Preliminarysep

5、arationAnexampleSmallmoleculesLargemoleculesMidium-sizemoleculesReferencesKremmer,T.(1979).Gelchromatography:theory,methodology,applications/byTiborKremmerandLászlóBoross.London;NewYork:Wiley.