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1、复旦学报(医学版)FudanUnivJMedSci2005Mar,32(2)139达菲抗原趋化因子受体在不同转移性人乳腺癌＊细胞中的差异表达■△■■■■魏思方王杰欧周罗李大强侯意枫罗建民邵志敏(复旦大学上海医学院生物化学与分子生物学系,■复旦大学附属肿瘤医院乳腺癌研究所上海200032)【摘要】目的研究不同转移潜能乳腺癌细胞株中达菲抗原趋化因子受体(Duffyantigenreceptorforchemokines,DARC)mRNA表达和蛋白质水平的差异,探讨DARC与乳腺癌细胞转移潜能的相关性。方法培养高低转移潜能的人乳腺癌MDA
2、-MB-435细胞株,并将其接种到裸鼠体内,以体外培养细胞和裸鼠体内原位异种移植瘤为标本,用RT-PCR法检测DARCmRNA的表达,同时在细胞涂片上,用抗Fy3单抗检测DARC的蛋白质水平。结果高转移潜能MDA-MB-435细胞的DARC表达显著性地低于低转移潜能MDA-MB-435细胞,并且在接种高转移潜能MDA-MB-435细胞而形成的裸鼠体内原位移植瘤中的DARC表达也显著性地低于低转移潜能MDA-MB-435细胞来源的移植瘤。结论本研究首次揭示趋化因子清除槽DARC的表达差异与人乳腺癌细胞的不同转移潜能有关,有望成为抑制乳腺
3、癌转移的新靶点。【关键词】达菲抗原趋化因子受体(DARC);趋化因子;乳腺癌转移【中国图书馆分类法分类号】R737.9ExpressionofDuffyAntigenReceptorforChemokinesinHumanBreastCancerCellLinesAssociatedwithDifferentMetastaticPotentialinvitroandinvivoWEISi-fang,WANGJie■,OUZhou-luo,LIDa-qiang■,HOUYi-feng■,LUOJian-min■,SHAOZhi-min■
4、(DepartmentofBiochemistryandMolecularBiology,ShanghaiMedicalCollege,FudanUniversity,Shanghai200032;■BreastCancerInstitute,CancerHospital,FudanUniversity,Shanghai200032,China)【Abstract】PurposeToobservetheexpressionofDuffyantigenreceptorforchemokines(DARC)inhu-manbreastca
5、ncercelllinesMDA-MB-435withdifferentmetastaticpotentialinvitroandinvivo.MethodsCulturedhumanbreastcancercelllinesMDA-MB-435withdifferentmetastaticpotentialandor-thotopicxenotransplantationtumorsinimmunodeficientnudemice.TheDARCmRNAexpressionandpro-teinlevelinthebreastca
6、ncercelllineswithdifferentmetastaticpotentialweredetectedbyreversetranscrip-tion-polymerasechainreactionandimmunohistochemistryrespectively.TheDARC-geneexpressioninortho-topicxenotransplantationtumorsinimmunodeficientmicewasdetectedbyreversetranscriptionpolymerasechainr
7、eaction.ResultsTheexpressionofDARCinbreastcancercelllineMDA-MB-435withhighmetastaticpotentialwassignificantlylowerthanthatofbreastcancercelllineMDA-MB-435withlowmetastaticpotential,samechangewasalsoobservedinorthotopicxenotranplantationtumorsinimmunodefi-cientmice.Concl
8、usionsOurresultssuggestedforthefirsttimethatdifferentexpressionofDARCisas-sociatedwithmetastaticpotentialofhum
