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1、CHAPTER1InteractionsBetweenAgaricusbisporusandthePathogenVerticilliumfungicolaPeterMills,JohnThomas,MartinSergeant,AnaCosta,PatrickCollopy,AndyBailey,GaryFosterandMikeChallenContents1.Introduction22.PathogenVariability43.Extra-CellularEnzymeProduction54.Screeningf
2、orResistance85.FungalTransformation96.MolecularInteractionsofVerticilliumandAgaricus107.FunctionalCharacterisationofHostPathogenResponseGenes128.FutureWork14Acknowledgements15References15AbstractTheinteractionbetweenAgaricusbisporusandVerticilliumfungicolamaybethe
3、mosteconomicallysignificantinteractionbetweentwofungi.Workde-scribedinthischaptercoversdiverseaspectsofthebiologyandmolecularinteractionofthehostandpathogenandhasestablishedarobustbaselineforfuturestudyofapathogenicsysteminvolvingtwomembersofthesamekingdom.Pathogen
4、variabilityhasbeenassessed,andspecificgenotypesimpor-tanttomajormushroom-producingcountrieshavebeencharacterised.Thishasenabledrepresentativeisolatestobeidentifiedforuseinthisstudy.Awiderangeofcell-wall-degradingenzymesfromV.fungicolahavebeenidentified,andprincipalco
5、mponentanalysisshowedacomplexcor-relationbetweenenzymeproductionandsymptomexpression.Itislikelythatsome,orindeedmanyoftheseenzymesplayacriticalroleinthepathogenicityofVerticillium.AlthoughitisgenerallyacceptedthatA.bisporushasarelativelynarrowgeneticbase,ourwork,a
6、ndthatofothergroups,hasshownthatvariabilityinhosttolerancetoinfectionwithV.fungicolaexiststoalargeextenttojustifyadetailedstudytoexploitit.WehavedevelopedBritishMycologicalSocietySymposiaSeriesr2008TheBritishMycologicalSocietyPublishedbyElsevierLtd.Allrightsreserv
7、ed.12PeterMillsetal.transformationtechnologiesforthemushroomA.bisporus(Challenetal.,2000;Leachetal.,2004;Fosteretal.,2004b;Burnsetal.,2005,2006),itspathogenV.fungicola(Ameyetal.,2002,2003)andotherpathogenicfungi(Rogersetal.,2004;Gewiss-Mogensenetal.,2006).Recentad
8、vancesingenesuppressiontechnologiesforthehostincludedeploymentofanti-senseandRNAihairpinconstructstodown-regulateendogenousA.bisporusgenes(Burns,2004;He
