资源描述：
《Two-photon laser scanning fluorescence microscopy using photonic crystal fiber》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、JournalofBiomedicalOptics9(5),922±927(September/October2004)Two-photonlaserscanning¯uorescencemicroscopyusingphotoniccrystal®berGailMcConnellAbstract.WereporttheapplicationofasimpleyetpowerfulmodularUniversityofStrathclydepulsecompressionsystembasedonphotoniccrystal®bersthati
2、m-CentreforBiophotonicsprovesonincumbenttwo-photonlaserscanning¯uorescencemicros-SIBS,27TaylorStreetGlasgowG40NR,Scotlandcopytechniques.Thissystemprovidesmorethanasevenfoldincreasein¯uorescenceyieldwhencomparedwithacommercialtwo-photonErlingRiismicroscopysystem.Fromthis,weinf
3、erpulsesofIRradiatonoflessUniversityofStrathclydethan35fsdurationreachingthesample.©2004SocietyofPhoto-OpticalIn-DepartmentofPhysicsstrumentationEngineers.[DOI:10.1117/1.1778734]JohnAndersonBuildingGlasgowG40NG,ScotlandKeywords:multiphoton;microscopy;¯uorescence;ultrafast;non
4、linear.E-mail:g.mcconnell@strath.ac.ukPaper03110receivedSep.3,2003;revisedmanuscriptreceivedJan.8,2004,andFeb.5,2004;acceptedforpublicationFeb.16,2004.1Introductionmadetoprecompensatefordispersionbyincludingprismsinahome-builtlaserresonator,deliveringpulsesof15.5fsMultiphoton
5、laserscanningmicroscopy~MPLSM!isawell-3establishedtoolfordeepandliveimagingofbiologicalduration,butthisapproachisin¯exiblewhenusedwithal-tissue.1Twotypesoflasersourcessuitedtotwo-photonexci-ternativecommercialresonators.tationexist,namely,continuouswave~cw!andultra-short-Toso
6、lvethisproblem,wedevelopedaneffectivepulsepulsed~USP!lasers,thelatterdeliveringpulsestypicallyofcompressiontechniquebasedonphotoniccrystal®ber~PCF!.4subpicoseconddurationeveryfewnanoseconds.TheresultingPreviousstudiesbyOuzounovetal.demonstratedthatul-peakpowerfromUSPlaserscan
7、easilyreachthekilowatttrashortpulsescanpropagatethroughPCF,butintheirinves-level,whileretainingamodestaveragepowerlevel.Theverytigationtheemittedpulsesarestretchedfrom100to140fs.highpeakpowersgeneratedbyUSPlasersourcesareidealforWereportonasystemthatcompressesthepulseduration
8、and,multiphotonapplicationswhenfocusedusinganobjectiveusingaconventi
