NVP对溶菌酶的吸附行为与吸附机理.pdf

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1、物理化学学报(WuliHuaxueXuebao)FebruaryActaPhys．-Chim．Sin．，2010，26(2)：359—366359[Article]www．whxb．pku．edu．cn接枝微球PMAA．HEMA／NVP对溶菌酶的吸附行为与吸附机理付红艳高保娇牛庆媛(中北大学化学工程系，太原030051)摘要：采用反相悬浮聚合法制备了甲基丙烯酸羟乙酯(HEMA)与Ⅳ_乙烯基吡咯烷酮(NVP)的交联共聚微球HEMA／NVP，然后采用“接出”法，实施了甲基丙烯酸(MAA)在交联微球表面的接枝聚合，制得了接枝微球PMAA—HEMA／NVP．以溶菌酶(LY

2、Z)为模型碱性蛋白，深入研究了接枝微球PMAA—HEMA／NVP对碱性蛋白的吸附性能与吸附机理．测定了微球PMAA—HEMA／NVP的zeta电位，考察了PMAA接枝度、介质pH值及离子强度等因素对体系吸附性能的影响．结果表明，在较大的pH范围内，接枝微球PMAA—HEMA／NVP的zeta电位为绝对值较大的负值，即其表面携带有高密度的负电荷．在强静电相互作用的驱动下，接枝微球PMAA—HEMA／NVP对溶菌酶表现出很强的吸附能力．随介质pH值的增高，接枝微球对溶菌酶的吸附容量呈现先增大后减小的变化趋势，在与溶菌酶等电

3、接近的pH值处(pH_9)，具有最大的吸附容

4、量(90mg·g)；离子强度对接枝微球的吸附能力也有较大的影响，当pH<9时，溶菌酶吸附容量随NaC1浓度的增高而减sb；当pH>9时，吸附容量随NaC1浓度的增高而增大．关键词：聚甲基丙烯酸；聚合物接枝微球；碱性蛋白质；溶菌酶；吸附机理中图分类号：0647AdsorptionBehaviorandMechanismofGraftedPMAA-HEMA／NVPMicrospherestowardLysozymeFUHong—YanGAOBao—JiaoNIUQing—Yuan(DepartmentofChemicalEngineering,NoahUniversi

5、tyofChina，Taiyuan030051，PR．China)Abstract：Thecrosslinkingcopolymerizationof2-hydroxyethylmethacrylate(HEMA)andN-vinylpyrrolidone(NVP)wasperformedusingtheinversesuspensionpolymerizationmethodresultingincrosslinkedHEMA／NVPmicrospheres．Subsequently，thegraftpolymerizationofmethacrylicacid

6、(MAA)wasconductedusingthe“graftingfrom”methodandthegraftedPMAA—HEMA／NVPmicrosphereswereobtmned．Theadsorptionpropertyandadsorptionmechanismofthegraftedmicrosphereswereinvestigatedindepthusinglysozyme(LYZ)asabasicproteinmode1．Thezetapotentialofthegraftedmicrosphereswasdetermined．Several

7、factorsthatafectedtheadsorptionpropertyofthesystemsuchasthepHofthemedium，thegraftingdegreeofthemicrospheresandtheionicstrengthwereexamined．ExperimentalresultsindicatethatinawidepHrange，thezetapotentialofthegraftedPMAA-HEMA／NVPmicrosphereshasalargenegativevalue．Therefore，ahighdensityof

8、negativechargeispresentonthesurfacesofthegraftedmicrospheres．ThestrongelectrostaticinteractionresultsinwhichthegraftedPMAA—HEMA／NVPmicrospheresexhibitverystrongadsorptionabilitytowardlysozyme．Theadsorptioncapacityofthegraftedmicrospheresforlysozymeinitiallyincreasesandthendecreasesast

9、hepHi