OT型百合品种黄天霸花器官组培快繁体系的建立.pdf

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1、南方农业学报JournalofSouthernAgrieuhure2014，45(5)：715—719ISSN2095—1191；CODENNNXAABDOI：10．3969／j：issn．2095—1191．2014．5．715TissuecultureandrapidpropagationofOThybridslilyManissafloralorgansNONGYan-feng1，ZHOUShan2，MOUYu—meil，CENXiang—ta01，SUXiao—huil．YANGMei—chun+(1AgriculturalCollege，GuangxiUnivers

2、ity，Nanning530005，China；2SugarcaneResearchInstitute，GuangxiAcademyofAgriculturalSciences／SugarcaneResearchCenter，ChineseAcademyofAgriculturalSciences／GuangxiKeyLaboratoryofSugarcaneGeneticImpr0Vement／KeyLaboratoryofSugarcaneBiotechnologyandGenetichnprovement(guangxi)，Ministry-ofAgriculture，

3、Nanning530007，China)Abstraet：【Objective】Theinvitrocultureandrapidpropagationoffloralorgansderivedf'olitOThybridslilyManissawasconductedtoprovideteehniealsupporlsonindustrialproductionofitsseed—bulbs．【Method】Diflerentparisofflowerbudsandhalf—openflowerofManissawereusedasexplantsandMSwasuseda

4、sbasecuhureriledi—Utit．Thecallusinductionefleetsofdiflerentexplantswereinvestigated．Theitlflueneesof0—3．0ing／L6一BAoncal—lusdiflerentiationcultureofexplantswerealsoanalyzed．aswellasdiflerentcombinationsof1．O一3．0ing／L6一BAand0—0．2mg／LNAAonadventitiousbudsinduetionderivedf'0111sterileleaves．30．

5、O一80．0ing／Lsugaronbulbletfomtationandrootingculture．Furlherntore，ditterentplantingsubstrateswereusedforplantlettransplanting．【Result】Thediflerentparisofflowerbudsandhalf—openflowerpresenteddiflerentcallusinduetionefleets．theovargshowedtheeasiestincallusinduction，followedbystigma．anther．rece

6、ptacle．filamentandpetal．Thecallusinductionratesofbothovaryandstigmawasover50．O％．InMSmediunt．thediflerentiationrateofcallusshowedinereasefrstlyandthendecreasewiththeinereaseof6一BA(O一3．0mg／L)．ThesuitablecallusdiflerentiationmedimnwasMS+2．5mg／L6一BA．Byaddingdiflerentcombinationsof6一BAandNAAinMS

7、．itfoundthatMS+3．0ing／L6一BAwasappropriateforinductionofadventitiousbudsderivedfrontsterileleaves．Adding60．0g／LsugarinMSmediuntcouldpronlotebulbletforotationandrooting．Theinducedplantletswithbulbletscouldsmwivebytransplantingtovegetablegardensoil．【Conclus