资源描述：
《Automated, Kinetic Imaging of Cell Migration andInvasion Assays using Corning® FluoroBlok™ Inserts细胞迁移地自动、动态成像 使用Curn宁®氟硼酸盐插入物地侵袭试验.pdf》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、Automated,KineticImagingofCellMigrationandInvasionAssaysusingCorning®FluoroBlok™InsertsApplicationNoteBradLarson1andHilarySherman2Reagents1BioTekInstruments,Inc.,Winooski,VTCellTracker™GreenCMFDADye(LifeTechnologiesCat.No.C2925).2CorningIncorporated,LifeSciences,Kennebunk,MECytatio
2、n™3CellImagingMulti-ModeReaderTheBioTekCytation3ImagingMultiModeReadercombinesIntroductionautomateddigitalmicroscopyandconventionalmulti-modeMetastasis,thespreadofadisease-producingagent(suchasmicroplatedetectionprovidingrichphenotypiccellularinfor-cancercells)fromtheprimarysiteofd
3、iseasetoanotherpartofmationandwell-basedquantitativedata.Withspecialempha-thebody,hasbeenassociatedwithapproximately90%ofcan-sisonlive-cellassays,Cytation3featurestemperaturecontroltocer-relateddeaths1.Therefore,itposesthesinglemostsignificant45°C,CO/Ogascontrolanddualinjectorsfork
4、ineticassays.The22challengetomanagethisdisease.Themetastaticprocessinvolvesinstrumentwasusedtoimagecellsmigratingorinvadingtothemigrationofcellsawayfromtheoriginaltumor(manytimesinbottomoftheCorning®FluoroBlok™membrane.responsetoaparticularregulatoryfactor),followedbyinvasionintosu
5、rrounding,ordistanttissue.Gen5™DataAnalysisSoftwareGen5softwarecontrolstheoperationoftheCytation3forbothWhilemanymethodsexisttomeasurethemigratory,andinvasiveautomateddigitalmicroscopyandPMT-basedmicroplatereading.characteristicsofcancercells,theadventofpermeablesupportsImageacquis
6、itioniscompletelyautomatedfromsampletransla-hasprovidedasimpleinvitroapproachtoperformtheseassays.tion,focusingtoexposurecontrol.MontagecapabilitiesallowforTheadditionofafluorescenceblockingmembraneimprovesthecaptureofimagesacrosstheentiresurfaceofalow-densitytheaccuracyoftheassaya
7、ndallowstheuseofinvertedfluores-platewell,suchasthe24-wellpermeablesupportsusedforthiscencemicroscopyforanalysis.Thisprovidestheabilitytoaccu-application.Finally,imageanalysisallowsforcellcountingonratelycountonlythecellsreachingthebottomofthemembraneeachimageandforreportingoftotal
8、resultsfromeachwell.instea
