果蝇的核小体定位与基因调控.ppt

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1、TheSchoolofLifeSciencesandTechnologyTongjiUniversity果蝇的核小体定位与基因调控江赐忠同济大学生命科学与技术学院2021年7月27日Whatisnucleosome?H2A,H2B,H3andH4AcetylationMethylationUbiquinationSumoylationPhosphorylationADPribosylationDeiminationProlineisomerizationDNANucleosomeChromosomeModifiedfromNature2008,4

2、54:711-715DNApackagingNucleosomeandgeneregulation红细胞神经细胞小肠表皮细胞脂肪细胞平滑肌细胞Onegenome,multipleepigenomeseggembryolarvapupaadultModelorganism:DrosophilaMethodsScreenshotofbrowserChromosome3LNature446(7135):572-6(2007)TSSAggregationofnucleosomepositionsNucleosomeorganizationaroundTS

3、Sgene+1+2+3+4+5NFRAggregationofnucleosomepositions123456789ABCDEFORFendNucleosomeorganizationaroundORFendgeneNFRNucleosomallandscapeofgenes5’NFR3’NFRNat.Rev.Genet.10(3):161-72(2009)PausedPolIIgene+1+2+3+4+5PausedPolIINotpausedvs.pausedgenesgene+1+2+3+4+5+1Nucleosomeshiftgene+

4、1+2+3+4+5gene+2+3+4+5PausedPolII1010101010PolII-boundnucleosomesPolIIYAbXLImmunoprecipitation&DNApurificationHybridizationtotilingarrayMappingprobestogenomePolII-boundnucleosomesgene+1+2+3+4+5PolIIDrosophilavs.yeastNFRgene+1+2+3+4+5-1GAGAmotifandGAFGAGAGGgene+1+2+3+4+5GAFNucl

5、eosomepositioningsequences(TSS)Nucleosomepositioningsequences(ORFend)NucleosomepackingNat.Rev.Genet.10(3):161-72(2009)NucleosomeduringreplicationMotifsdistributionaroundnucleosomesModifiedfromChromosomeResearch(2006)14:5-16ModifiedfromNature(2007)446:572-576genemotifnucleosom

6、eArrangementofmotifsaroundTSSandnucleosomesGenome-widemotifarrangementNucleosomalAnti-nucleosomalFixedRandomgenemotifnucleosome-500bp-500bp+500bp10-1ConclusionUniformnucleosomearrangementingenicregionsAnNFRexistsatbothendsofgenesGAFmaycontributetolackof-1nucleosomesRNApolymer

7、aseIIengagesthe+1nucleosomeandpausesNon-randommotiforganizationrelativetonucleosomesDr.FrankPughDr.IstvanAlbertTravisMavrichLynnP.TomshoDr.IlyaIoschikhesDr.XiaoyongLiOtherlabmembersAcknowledgements同济大学生物信息系全职正教授曹志伟：中医药信息学和免疫信息学40余篇SCI论文：PharmacologicalReviews,Naturereviewsdru

8、gdiscovery,PNAS，MCP,NAR等。江赐忠：基因组学和表观遗传组学Nature(2008),GenomeRes(2008)