西罗莫司处理制备新型小口径异种移植血管.pdf

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1、ShanghaiMedJ，2013，Vo1．36，No．11●论著●西罗莫司处理制备新型小口径异种移植血管王学宁陈长志顾严己张顺业李保奚吉成【摘要】目的将经脱细胞和肝素处理的小口径异种移植血管结合西罗莫司，旨在进一步改进这种移植血管的性能。方法取新鲜成年犬的颈动脉，经脱细胞和肝素结合处理后分为对照组(经脱细胞和肝素处理)和西罗莫司组(脱细胞和肝素联合西罗莫司处理)。取新西兰大白兔2O只，将对照组和西罗莫司组的血管分别移植入同一只兔的左、右侧颈动脉。术后1、2、4、8周采血，采用反相高效能液相层析法

2、(HPLC)检测西罗莫司组中西罗莫司的含量。术后3个月通过超声检查观察移植血管的通畅情况。术后3和6个月分别随机处死1O只兔，取双侧血管样本经免疫组织化学和苏木精一伊红(H—E)染色观察血管重塑和内膜增生情况。结果西罗莫司组术后l、2、4、8周血管中均可检测到西罗莫司。术后3个月，对照组有5根移植血管内有斑块形成，斑块发生率为5／ZO，显著高于西罗莫司组的2／20(P~0．05)。术后3和6个月H—E染色显示，两组部分血管管腔内有血栓形成和内膜增生，对照组吻合口近、远端的内膜厚度和吻合口远端的内膜

3、与中膜厚度比值均显著高于西罗莫司组同时间点(P值分别

4、muscoatingWANGXuening，CHENChangzhi，GUYanji，ZHANGShunye，LIBao，XlJichengDepartmentofCardiovascularSurgery，ShanxfCardiovascularHospita『，丁aiyuan030024，Shanxf，ChinaCorrespondingauthor：WANGXueningE—mail：wangxuening一2004@126．corn[Abstract]ObjectiveTodevelopa

5、newsmall—caliberdecellularizedandheparinizedvascularxenograftthroughsirolimustreatment．MethodsCaninecarotidarterieswerechosenasxenograftsthroughdecellularizationandheparintreatmentThexenograftswith(n20，controlgroup)andwithout(n20，sirolimusgroup)siroli

6、muscoatingwereimplantedinrabbits’leftandrightcarotidartery，respectively．Thecontentofsirolimuswasmeasuredbyhighperformanceliquidchromatography(HPLC)at1，2，4，and8weeksinsirolimusgroup．GraftpatencywascheckedbyDuplexuIlras0nOgraphyat3monthsafterimplantatio

7、n．Tenrabbitsweresacrificedrespectivelyat3and6monthsafterimplantationandbilateralgraftswereexplanted．ImmunohischemicalstainingandHematoxylin—Eosin(H—E)stainingwereusedtoobservevascularremodelingandintimahyperplasia(IH)ofbilateralgrafts．ResultsSirolimus

8、wasfoundinvascularwallofsirolimusgroupduring8weeksafterimplantation．At3monthsafterimplantation，plaqueformationwasfoundin5bloodvesselsofcontrolgroup(5／2O)butin2bloodvesselsofsirolimusgroup(2／20，P<0．05)．At3and6monthsafterimplantation，H-Estaining