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1、LabonaChipOr:TheIncredibleShrinkingScienceExperiment!Or:APOIBE!Credits:MathiesLab,UC-BerkeleyQuakeLab,StanfordAgilent,Inc.二十一世纪科学发展趋势MiniaturizationAutomationIntegrationPortableLabonaChip?MicrofluidicsMicrofluidics:TheuseofmicrofabricationtechniquesfromtheICindustrytofabricatechann
2、els,chambers,reactors,andactivecomponentsonthesizescaleofthewidthofahumanhairorsmallerCredit:Dr.KarenCheung,UBCECEWhyusemicrofluidics?1moleculein1mL=1.6x10-18M1moleculein1nL=1.6x10-15M1moleculein1pL=1.6x10-12MCredit:Dr.KarenCheung,UBCECESamplesavings–nLofenzyme,notmLFasteranalyses–
3、canheat,coolsmallvolumesquicklyIntegration–combinelotsofstepsontoasingledeviceNovelphysics–diffusion,surfacetension,andsurfaceeffectsdominateThiscanactuallyleadtofasterreactions!Whyusemicrofluidics?1-3min/12samplesMulti-channelmicrochipelectrophoresis1-3minMicrochipelectrophoresisd
4、etectionHighvoltagebuffersamplecapillary10-30minCapillaryelectrophoresisseparationbuffer1-3ｈGelelectrophoresisSomeinterestingLOCapplicationsGeneticanalysisPortabledevicesforpathogendetectionHigh-throughputbiologyChemicalsynthesisCellImmunoassayanalysisMetaboliteanalysisDrugdelivery
5、systemsSinglemolecularEnzymeanalysisCourtesyofDr.BorisStoeberGeneticanalysismicrosystems96-channelradialcapillaryarrayelectrophoresismicroplateAnalChem.1999;71(23):5354-61BacterialPathogensFoodpoisoningWoundinfectionsBiowarfareFoodpoisoning:PathogenicE.coli:>1in10,000inCanadaSalmon
6、ellaspp.:ashighas1in50eggsWoundinfections:Antibiotic-resistantS.aureus:ashighas50%LungDisease:Pseudomonasspp.MycobacteriumtuberculosisBiowarfare:Anthrax,plague,smallpoxLungDiseaseDr.SharonPeacock,UniversityofOxfordMethodsforGeneticDetectionofPathogensOLDWAY:NEWWAY:Developbacterialp
7、athogendetectorsthatare:Rapid(<1hour)Sensitive(detectasinglecell)GeneticallySpecificField-portableGeneral(manydifferenttypesofpathogens)PCR-CEMicrosystemFabricationPortableSystemforInfectiousDiseaseDetectionSystemmeasures8x10x12inchesCompletefluorescencedetectionincluding488-nmsoli
8、d-statelaser,PMT,optics,an
