GeneXpert技术及原理教学内容.pptx

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1、GeneXpert技术及原理马林Feb2016真正的开始2©Cepheid–ProprietaryandConfidential分子诊断检测（PCR）包含以下步骤：样本采集仪器平台,耗材&软件样本处理扩增检测报告结果SmartCyclerSystem1©Cepheid–Proprietary&ConfidentialGeneXpertSystem©Cepheid–ProprietaryandConfidentialSmartCyclerSmartCyclerII6©Cepheid–ProprietaryandConfidentialS

2、martCycler反应管25l不会损坏！7©Cepheid–ProprietaryandConfidential最优化的设计:快速温度响应最佳的荧光信号预防污染一次性反应管LargeSurfaceTemperatureAreawiththinwallenclosureSealablepressurecapPolishedOpticwindows(with90°opticspath)OpticReflectors8©Cepheid–ProprietaryandConfidentialPAGE9

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4、ryandConfidential9DisposableTubecompensatesClumsiness固定的组件PAGE12

5、©Cepheid–ProprietaryandConfidential12BioRadQIAGENRoche系统模块任意配置MultipleI-COREModulesAsSystemBuildingBlocks11©Cepheid–ProprietaryandConfidentialSmartEBVFCTC灵活性:荧光及模块SmartCMVFCTCASRHSVFATAASREVFATA12©Cepheid–

6、ProprietaryandConfidentialMasterMixforaPCRassayBufferMgCl2TaqPolymerasedNTPmixturePrimersProbeICPCR反应13©Cepheid–ProprietaryandConfidential扩增/检测SmartMixTMHM预混珠14©Cepheid–ProprietaryandConfidentialReconstitutewithWaterAddthesampleStarttheRun预混珠形式避免加样误差15©Cepheid–Proprieta

7、ryandConfidential诊断软件:DX3.03AssayTypesResearchReferenceIVD16©Cepheid–ProprietaryandConfidential©Cepheid–ProprietaryandConfidentialGeneXpert系统GeneXpert技术18©Cepheid–ProprietaryandConfidential整合的微流体技术，包括：仪器平台试剂盒运行程序和对照实时荧光定量PCR(扩增和检测同时进行)仪器和样品盒之间不存在湿式接触界面，以消除污染反应体系完全内部质控-不

8、需要设置独立的外部阳性和阴性对照集成的细胞超声裂解释放DNA的装置GeneXpert软件指示单个模块主板以协调阀驱动和整体液压传动精巧的流体学技术液体流动由微型阀控制适于试剂成分的微量反应全自动的数据分析和结果解读19©Cepheid–ProprietaryandConfidentialGeneXpert设计赛沛的技术洗涤DNAorRNA提取扩增检测分析阳性阴性DNAorRNA纯化患者样本PCR反应体系SmartCyclerGeneXpert20©Cepheid–ProprietaryandConfidential赛沛的技术Smart

9、CyclerGeneXpert21©Cepheid–ProprietaryandConfidentialI-Core模块模块化设计的SmartCycler和GeneXpert22©Cepheid–ProprietaryandConfidentialGeneXpert6色荧光通道23©Cepheid–ProprietaryandConfidential通道激发波长范围接收波长范围荧光染料1375-405nm420-480nmDye12450-495nm510-535nmDye23500-550nm565-590nmDye34555-59

10、0nm606-650nmDye45630-650nm665-685nmDye56630-650nm>700nmDye6仪器平台I-CORE模块SmartCycler®GeneXpert®GeneXpert模块BDS24©