烟草野火病病原菌对农用链霉素的抗药性测定

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1、烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定烟草野火病病原菌对农用链霉素的抗药性测定?328?ChineseAgriculturalScienceBulletinVo1.23No.122007Decemberhttp://www.casb.org.cn烟草野火病病原茵对农用链霉素的抗药性测定李梅云(云南省烟草科学研究所,玉溪653100)摘要:为合理防治烟草野火病,减少抗药性产生,减少农药残留.从云

2、南省8个地州分离烟草野火病茵茵株43个,并就分离茵株对农用链霉素的抗药性进行了测定,结果表明,供试43个茵株中高抗茵株8个,中抗茵株比例较高17个,低抗茵株18个.不同来源的烟草野火病原茵株的抗药性存在差异,文山,昆明的茵株抗药性最高,红河茵株抗药性最低,抗性倍数最高达2000多倍,其余地州抗药性居中.不同地州分离菌株高,中,低抗茵株的比例存在明显差另l】.分离自文山邱北县双龙营镇的25#茵株抗药性最强.抗性倍数达45907.75,来自文山砚山县阿猛镇租白村的34#茵株抗药性最低.43株烟草野火病茵茵株对农用链霉素的EC50值平均为4.65,范围在0.004~183.631.高,中,低抗茵株

3、的平均抗性倍数分别为6124.69,49.72,6.40.关键词:烟草野火病;农用链霉素;抗药性中图分类号:$572文献标识码:ADeterminationofPseudomonassy~ngaepv.tabaciResistancetoAgriculturalStreptomycinLiMeiyun(TobaccoScienceResearchInstitute,YunnanYuxi653100)Abstract:Tocontroltobaccowildfirediseaserationally,reduceresistanceandreducepesticidepollution,43s

4、trainsofPseudomonassyringaepv.tabaciwereisolatedfrom8regionofYunnanprovinceandalli8o—latesweredeterminedtheirresistancetoagriculturalstreptomycin.Theresultsindicatedthatofalltestediso—lates,therewere8highresistance,17middleresistanceand18lowresistanceisolatesrespectively.I’}leis1).-latesfungicidere

5、sistancefromdifferentregionwasdifferentobviously,thehighestresistantisolatecomefromWenshanandKunmingregion.Hongheisolateshadthelowestresistanceamongalltestedisolates.I1leevi-dentdisparityofhigh—resistance.mid—resistanceandlow—resistanceisolatesratiowasexistatdifferentre—gion.25#fromWenshanQiubeiwas

6、thehighestagriculturalstreptomycinresistantisolate,itsresistancemultiplearrivedto45908comparedtothelowestresistanceisolate34#,whichfromYanshanofWenshan.I1leEC50of43Pseudomonassyringaepv.tabaciisolatesagainstagriculturalstreptomycinwas4.65inaver-age.wasintherangeofO.004-183.631.,I1leaverageresistanc

7、emultipleofhigh—resistance,mid—resistanceandlow-resistanceisolateswas6124.69,49.72,6.40respectively.Keywords:tobaccowildfirediseases,agriculturalstreptomycin,fungicideresistance烟草野火病是由烟草假单胞杆菌(Pseudomonassyr