鸡cacna1s 基因snps 及其与部分屠体性状的关联性研究

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1、鸡CACNA1S基因SNPs及其与部分屠体性状的关联性研究张军（温州科技职业学院，浙江温州325006）摘要：研究发现L型钙离子通道α1亚单位（CACNA1S）与动物肌肉的收缩和发育存在一定的关系，但还未见鸡CACNA1S基因的研究。利用PCR-SSCP技术，对萧山鸡、仙居鸡、灵昆鸡、萧山鸡×仙居鸡杂交F1代、爱拔益加鸡CACNA1S基因5′-调控区（5′-UTR）和外显子1（exon1）首次进行多态性检测，结合屠体性状做关联分析。结果表明试验群体CACNA1S基因5′-UTR和exon1存在2个单核苷酸多态性（SNPs）位点与屠体性状关联：SNPs716位点JJ基因型的胸肌率极显著（P

2、<0.01）大于KK基因型。SNPs1711位点YY基因型的全净膛率和胸肌率显著大于（P<0.05）XY和XX基因型；YY和XY基因型的半净膛率和胸肌率极显著（P<0.01）大于XX基因型。初步推断CACNA1S基因可能是影响鸡部分屠体性状（全净膛率、半净膛率、胸肌率、腿肌率）的主效基因或与主效基因连锁，并且这些突变位点可能被用作分子遗传标记选择鸡的屠体性状。关键词：鸡；CACNA1S基因；PCR-SSCP；屠体性状；关联分析StudyonAssociationofSingleNucleotidePolymorphismsofCACNA1SGenewithPartialCarcassTr

3、aitsinChickenZhangjun(Wenzhouvocationalcollegeofscience&technology,Wenzhou325006,China)Abstract:Calciumchannel,voltage-dependent,Ltype,alpha1Ssubunit(CACNA1S)wasrelatedtothecontractionandgrowingdevelopmentofmuscle,buttherewerenotthestudyofCACNA1Sgeneinchicken.Singlenucleotidepolymorphismof5′-Untr

4、anslatedRegions(5′-UTR)andExpressedregion1(exon1)ofCACNA1Sgeneof30Xiaoshanchickensand30Xianjuchickensand28Linkunchickensand30F1offspringofXiaoshanandXianjuchickensand24AAchickenswasanalyzedbyPCR-SSCP，anditsassociationanalysiswithcarcasstraitswasconductedforthefirsttime.Inresults,itwasfoundthat2Si

5、nglenucleotidepolymorphisms(SNPs)of5′-UTRandexon1ofCACNA1Sgene，anditswereassociatedwithcarcasstraitsoftheexperimentgroup.InthelocusSNPs716,BreastmusclerateofgenotypeJJwasverysignificantlybetterthanthatofgenotypeKK（P<0.01）.InthelocusSNPs1711,EvisceratedweightrateandbreastmusclerateofgenotypeYYwass

6、ignificantlybetterthanthatofgenotypeXYandXX（P<0.05）,Semi-evisceratedweightrateandbreastmusclerateofgenotypeYYandXYwereverysignificantlybetterthanthatofgenotypeXX（P<0.01）.ItwasconcludedfromtheresultsthatCACNA1Sgenemaybethemajorgeneaffectingcarcasstraits(Evisceratedweightrate、Semi-evisceratedweight

7、rate、breastmusclerate、legmusclerate)ofchickenoritlinkswiththemajorgene，andthemutationlocuscouldbeusedasmoleculargeneticmarkerstoselectthechickensforcarcasstraits．Keywords:chicken;CACNA1Sgene;PCR-SSCP;carcasstraits;corr