大葱油对胃癌细胞株体外培养的影响

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1、大葱油对胃癌细胞株体外培养的影响【摘要】　　目的：探讨大葱油对胃癌细胞的影响以及诱导胃癌细胞凋亡的机制.方法：MTT法评价大葱油对胃癌细胞增殖的影响.荧光染色观察细胞形态学变化.流式细胞术和放免法检测细胞凋亡及细胞内游离Ca2+和环磷酸腺苷（cAMP）浓度.结果：随着大葱油浓度的增加，作用24h后，细胞增殖抑制率分别达到5.71%，36.33%，44.34%，54.52%和74.15%，IC50≈65μg/mL.荧光显微镜下观察结果显示，实验组出现细胞凋亡形态学变化.100μg/mL大葱油作用于胃癌细胞1~12h，细胞

2、总凋亡率分别为50.16%，53.48%，77.84%，83.71%和41.34%；早期细胞凋亡率分别为50.15%,33.25%,11.69%，4.65%和0.84%.100μg/mL大葱油作用于胃癌细胞5min~12h，各相应时间点细胞内游离Ca2+含量依次为(71.00±6.08)，(77.67±1.53),(79.67±1.53)，(83.33±5.03)，(76.00±6.06)，(75.67±2.52)，(72.33±4.24)和(65.00±2.00)nmol/L；细胞内cAMP浓度依次为(5.76±0.

3、36),(18.51±2.06)，(20.40±1.65)，(16.09±1.46)，(12.16±0.78)，(9.88±0.53)，(9.47±0.78)和(9.82±1.06)pmol/mg（P<0.05).结论：大葱油可抑制胃癌细胞增殖，并通过上调细胞内Ca2+和cAMP水平诱导其凋亡.【关键词】大葱油；胃肿瘤；肿瘤细胞，培养的；凋亡；Ca2+；cAMP12　　EffectsofAlliumfistulosumL.oiloninvitrocultureofgastriccancercellline　　【A

4、bstract】AIM:ToinvestigatetheeffectsofAlliumfistulosumL.oil(ALFO)ongastriccancercelllineandthepossiblesignalmolecularmechanismunderlyingapoptoticprocessinducedbyAFLO.METHODS:HumangastriccancerMGC803cellswereculturedinvitroandtreatedbyAFLO.Thenitsgrowthinhibitory

5、ratewascountedandIC50wasmeasured.Apoptoticprocesswasinvestigatedwithfluorescencemicroscope.Intracellularfreecalciumcontentatdifferenttimeswasdeterminedbyflowcytometry.Intracellularcyclicadenosinemonophosphate(cAMP)levelsweredeterminedbyradioimmunoassay(RIA).RE

6、SULTS:WithincreasingtheconcentrationofAFLO,cellgrowthinhibitoryrateswentupcorrespondingly24haftertreatmentwithAFLOandreached5.71%,36.33%,44.34%,54.52%and74.15%respectively.IC50≈65μg/mL.Hoechst33258fluorescentstainingshowedthatapoptosisappearedinsomecellstreatedb

7、yAFLO(100μg/mL).WhenMGC803cellswereincubatedinthepresenceofAFLO(100μg/mL)from1to12h,thetotalapoptoticrateswere50.16%,53.48%,77.84%,83.71%and41.34%,respectively,andtheearlyapoptoticrateswere1250.15%,33.25%,11.69%,4.65%and0.84%,respectively.InMGC803cellstreated

8、byAFLO(100μg/mL)for5minto12h,freecalciumcontentswere(71.00±6.08),(77.67±1.53),(79.67±1.53),(83.33±5.03),(76.00±6.06),(75.67±2.52)，(72.33±4.24)and(65.00±2.00)nmol/L,re