资源描述：
《地灵愈伤组织到成体的发展研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、河南科技大学本科生毕业论文银条茎尖的脱毒组织培养和植株再生摘要银条（StachysfloridanaSchuttl.exBenth），又名地灵，系唇形科水苏属多年生蓄根草本植物，其地下根状茎圆状细长，肉质洁白，酷似白银，故名银条；据传是唐僧取经过程中所得，又被誉为“唐僧肉”。目前，全国人工种植面积很少，洛阳偃师县（市）是主要产地，约占全国产量的95%，仅春节左右大量上市，同时远销国内外，在市场上常供不应求[2]。本研究通过对银条茎尖分生组织的培养条件、不同处理对提高茎尖再生植株脱毒率的效果进行了研究，并利用不同外植体探讨了银条再生快繁的适宜途径。试验结果表明：银条的离体培养以MS作为基本
2、培养基最为适宜，再生苗生长快速，植株健壮。茎尖分生组织培养在MS+6-BA0.5mg/L+NAA0.1mg/L+蔗糖4％+琼脂6.5g/L上，最适宜茎尖的培养；再生植株的脱毒率随茎尖切割长度的减小而提高，但过小的茎尖操作、培养困难，培养周期长，成苗率低。0.5mm大小的茎尖成苗率与脱毒率相对较高，较为适合；茎尖的重复切割培养与接种植株进行预热处理均有显著提高脱毒率的作用，但同时也降低了成苗率。关键词：银条茎尖培养脱毒植株再生MS培养基III河南科技大学本科生毕业论文STACHYSFLORIDANAMREISTEMCULTUREANDPLANTLETREGENERATIONOFVENOMT
3、ISSUEREMOVALABSTRACTStachysfloridana,alsoknownasLing,LamiaceaeStachysisabuildrootperennialherb,itsundergroundrhizomesroundedslender,whitemeat,resemblessilver,hencethenameStachysfloridana;AccordingtolegendJourneytotheWest.,alsoknownasthe"officialtoldus.Theplantedareararely,LuoyangYanshicounty(city
4、)isthemainproducer,accountingforabout95%ofnationaloutput,alargenumberoflistedonlyaroundtheSpringFestival,andsoldathomeandabroad,ofteninshortsupplyinthemarket.Inthisstudy,onStachysfloridanaapicalmeristemcultureconditions,differenttreatmentstoimprovetheeffectofshoottipsregeneratedplantsdetoxificati
5、onrateanddifferentexplantssuitablewaytoexploretheStachysfloridanarenewablerapidpropagation.Theresultsshowed:AsthebasicmediumformanyflowerBetonyculturationinvitro,MSwasthebestinallmediaincludeWhite,MA,B5andNe.TheoptimalmediumfotthetipmeristemculturewasMS+6-BA0.5mg/L+NAA0.1mg/L+sucrose4%,onwhich6.5
6、g/Lplantletratiowasproduced.Theresultsshowedthatthesizeofexcisedshoottipwasnegativecorrelatedwiththevirus-freerateofplantlet.Toosmallershoottipmakeoperationandregenerationdifficult.Theattemptofusing0.5mmlengthshoottipwassuccessed.Thevirus-freeratecouldbeincreasedmarkedlybyusingthetipmeristemcultu
7、rationtechniquetogetherwiththetechniquessuchascutofstemtipmorethanonetimes,highertemperaturepre-treatmentoftheplanrmaterial.Howeverthesurvivalratemightbedegraded.KEYWORDS:Stachysfloridana，Stemtipculture，detoxication，Pl
