紫杉醇减少脊髓损伤后疤痕形成并促进轴突再生 ppt课件

《紫杉醇减少脊髓损伤后疤痕形成并促进轴突再生 ppt课件》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、Title：MicrotubuleStabilizationReducesScarringandCausesAxonRegenerationAfterSpinalCordInjuryFaridaHellaletal.Science331,928(2011);DOI:10.1126/science.1201148Reporter:***Supervisor:***SecondarySupervisor:***BackgroundsHypertrophicscarringandpoorintrinsic

2、axongrowthcapacityconstitutemajorobstaclesforspinalcordrepair.Microtubuledynamicsregulatekeyprocessesduringscarring,includingcellproliferation,migration,anddifferentiationaswellasintracellulartraffickingandsecretionofextracellularmatrix(ECM)molecules(1,

3、2).Moreover,moderatemicrotubulestabilizationpreventsaxonalretractionandswellingoftheaxontipaftercentralnervoussystem(CNS)injury(3)andstimulatesaxongrowthofculturedneurons(4),enablingthemtoovercomethegrowthinhibitoryeffectofCNSmyelin(3).AssumptionsTheyhy

4、pothesizedthatmoderatemicrotubulestabilizationwithTaxol,anapproveddrug,wouldfacilitateaxonalregenerationafterspinalcordinjury(SCI)bydecreasingscarformationandenhancingintrinsicaxonalgrowth.MethodsTheyfirstexaminedwhetherTaxoltreatmentreducedscarringafte

5、rSCI.Adultratsunderwentadorsalhemisectionattheeighththoracicspinalcordlevel;Taxol(256ng/day)wascontinuouslydeliveredattheinjurysiteusinganintrathecalcatheterconnectedtoanosmoticminipump.Whathappened7dayslater？FaridaHellaletal.Science2011;331:928-931Publ

6、ishedbyAAASFig.1Taxoldecreasesscarringinducedbyspinalcordinjury.(A)Representationoflesionedspinalcord(box).(BandC)Midsagittalsectionsoflesionsitefromratstreatedwith(B)vehicleor(C)Taxol(256ng/day)7daysafterinjury.Scalebars,300μm.(D)Taxolsignificantlydecr

7、easesfibroticscarring(expressedaspercentageofvehiclecontrol;n=12ratspergroup;**P=0.002;two-tailedttest)withoutaffectingglialcompaction(28daysafterinjury)(E)orinjurysize(F)(n=7to10ratspergroup;P=0.951;two-tailedttest).Dataexpressedasmean±SEM.GFAP,glialfi

8、brillaryacidicprotein.FigureS1:Taxoldecreasesfibronectin,NG2proteoglycanandcollagenIVaccumulationinducedbyspinalcordinjury(A)Representationoflesionedspinalcord(box).(B-D)Mid-saggitalsectionsoflesionsitefromrattreatedwithVehicleo