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1、重组细菌转座酶86Conclusionliposomesinbiochemistry[5]Reconstitutionofmembraneproteinsintoliposomesisapoechanismofactionofmembraneproteins.Asshoagic,’’andtheprospectsofachievingoptimalproteoliposomereconstitu-tionareobviouslygoodethodsandsystematicexperimentalanalysis.Thefutureofmembr
2、aneproteinreconstitutionappearsbrightinthelightofthesteadilyexpandingnumberofmembraneproteinsthathavebeenidentiedinthesequencingofthegenomesofdifferentorganisms.Poethodsofoverexpressionofgenesarenoembraneproteins.Inthiscontext,althoughnotlargelyexploiteduptonoembraneproteinsi
3、ntoliposomesshouldbeanefcientstrategyforthepurposeofpharmaceutical,cosmetic,andchemicalapplications(e.g.,specictargeting,drugdelivery,andantigenicityorgeherapy).[5]ReconstitutionofPurifiedBacterialPreproteinTranslocaseinLiposomesByChrisvanderDoes,JeaninedeKeyzer,MartinvanderL
4、aan,andArnoldJ.M.DriessenIntroductionThelastdecadehasseenamajoradvanceinthestudyofbacterialproteintranslocation(forrevieem-braneproteinsaresynthesizedattheribosomeinalsignalsequenceorhydrophobictransmembranesegmentsthatdirecttheproteinstothebacterialinner(cytoplasmic)membrane
5、.Manyinnermembraneproteinsaretargetedtothemembraneasaribosome-boundnascentchainviathesignalrecognitionparticle(SRP)andFtsY,theSRPreceptor.Thetargetingofmostperiplasmicandoutermembraneproteinsoccursposttranslationallyasapletelysynthesizedpolypeptide,ofteninasso-ciationolecular
6、chaperoneSecB.1Atthemembrane,both12E.H.MantingandA.J.M.Driessen,Mol.Microbiol.37,226(2000).A.J.M.Driessen,E.H.Manting,andC.vanderDoes,Nat.Struct.Biol.8,492(2001).Copyright2003,ElsevierInc.Allrightsreserved.0076-6879/03$35.00METHODSINENZYMOLOGY,VOL.372[5]preproteintranslocasei
7、nliposomes87targetingroutesconvergeatthetranslocase.3TranslocaseconsistsofaplexoftheATP-drivenmolecularmotorSecA4andaheterotrimericmembraneproteinplexposedoftheSecY,SecE,andSecGsubunits.5TheSecYEGplexformsaprotein-conductingchannelinthemembrane.6SecAisadissociablesubunitofthe
8、translocasethatasso-ciatesolecularmotorthatfuelsthetranslocationreac
