不同植物来源纤维素酶解产糖中构效关系及预处理影响研究

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1、华中农业大学2014届硕士研究生学位论文miscanthus>waterpeanut>metasequoiachips．Inanyofthecellulaseloadings(7—200FPU／gcellulose)，cellulosefrommetasequoiachips、^，itllthehighestcrystallinityindexhadtheminimumreducingsugaryield(20—60∥1009)，meanwhile，thesugarcanebagassecellulose谢tllthelowestcrys

2、tallinityindexhadthemaximumreducingsugaryield(>85．72g／1009)．Thehigherthecrystallinityindexwas，thehigherdegreeofcrystallizationwouldbe，andthemoredifficultthecombinationofenzymeandcelluloseWas，leadingtoalowercelluloseconversionandalowerreducingsugaryield．Anobviousnegativeco

3、rrelationbetweenCrIandhydrolysisyieldwasobtained、析tllthelinearcorrelationcoefficientRashighasO．9828，atsufficientlyhighenzymeloadingsof140and200FPU／gcellulosewheretheenzymeavailabilitywasnotalimitingfactorofthehydrolysisreaction．(3)Itwasfoundthatthecrystallinityindex(CrI)o

4、fplantcelluloseplayedagreaterrolethandegreeofpolymerization(DP)andspecificsurfacearea(SSA)did，inenzymehydrolysisbythemultiplelinearregressioncorrelationequationfittingbetweenthereducingsugaryield(Ⅵandstructuralcharacteristics(CrI，DP,SSA)，meanwhile，theDPandSSAonlypresented

5、aminordegreeofpositivecorrelation、析tllthereducingsugaryield，thushadaminorinfluenceonthehydrolysisofplantcelluloses．TheinfluenceofstructuralcharacteristicsonenzymatichydrolysisWasintheorderofCrl>DP>SSA．Thispointstothepotentialadvantagesinpracticalapplicationofutilizingwate

6、rhyacinthasfeedstockforbio—ethanolproductionasithasrelativelylowCrIandrelativelyhighSSA．Whenwaterhyacinthisappliedtoenzymatichydrolysis，itCannotonlyreducethecostofenzymereagentaswellasthecostofbio-ethanolproduction,butalsocontroltheaquaticenvironmentalproblems．(4)Differen

7、tintensityofphosphoricacidandmicrowavepretreatmentswereemployedtowaterhyacinthcelluloseseparatedbyethanol—nitratemethodinordertochangeitsstructttralfeature．TheSSAofthepretreatedmaterialsdecreasedsignificantly,andthereducingsugaryieldsofthesematerialswerealsolowerthanthose

8、ofuntreatedcellulose．Therefore，waterhyacinthcelluloseseparatedbyethanol—nitratemethoditselfwasal