资源描述：
《英文-学术报告》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、CharacteristicsofEscherichiacolibiofilmproduction,genetictyping,drugresistancepatternandgeneexpressionunderaminoglycosidepressureswww.themegallery.comEscherichiacoli大肠杆菌infection感染Aminoglycoside氨基糖苷类antibiotic抗生素Pseudomonas假单胞菌aeruginosa聚合酶链反应技术Crystalvioletstainin
2、gmethod结晶紫染色法phenotype表现型drugresistancepattern耐药模式Antimicrobialagent抗菌药物molecularbiologyreagent分子生物学试剂Brothmicrodilutionmethod肉汤稀释法Susceptibilitytesting药敏试验Genotype基因型Real-timeqRT-PCR实时定量荧光PCRStreptomycin链霉素amikacin丁胺卡那霉素Gentamycin庆大霉素apramycin安普霉素1.Newwords,www.t
3、hemegallery.comBackgroundSeveraltypesofinfectioncausedbyEscherichiacoli(e.g.,urethralcatheter,biliarytractprosthesis,biliarytractinfection,lithangiuria,urinarytractinfectionandmanyotherdiseases)areassociatedwithbiofilmformation,whichleadstoaninabilitytoeradicatethe
4、infectionduetoitsintrinsicnaturetoresisthighlevelsofantibiotics(FontaineandSmith,2006;Melchioretal.,2006)..www.themegallery.comBackgroundInaddition,underextensiveandpersistentpressureofantibiotics,inordertosurvive,theE.coliformsbiofilmtoevadetheimmuneclearanceandre
5、sultsinmultipleantibioticsresistance.Hoffmanetal.(2005)foundthatsubinhibitorylevelsoftheaminoglycosideantibioticscouldinduceE.coliandPseudomonasaeruginosabiofilmformation.SomeotherstudiesalsorevealedthatbiofilmformationbyE.coliinvitrocorrelatedwiththevirulencefactor
6、s(Navesetal.,2008;Rijavecetal.,2008).www.themegallery.comAbstractInthisstudy,qualitative(scanningelectronmicroscope)andsemi-quantitative(modifiedcrystalvioletstainingmethod)methodshadbeenusedtoevaluateEscherichiacolibiofilm-formingability.Brothmicrodilutionmethodand
7、enterobacterialrepetitiveintergenicconsensus-basedPCR(ERIC-PCR)wereperformedtostudyE.colidrugresistancepatternandgenetictyping.Basedontheresultsabove,westudiedthecorrelationbetweenbiofilm-formingabilityphenotype,drugresistancepatternandgenetictypinginE.coli.Real-ti
8、meqPCR(qRT-PCR)wasusedtorevealmRNAexpressionlevelofE.colibiofilmrelatedmultipleantibioticsresistancegenes(acrA,agn43,csgA,csgD,ompFandpgaA)underdifferent
