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1、MS培养基的配制与灭菌PreparationandsterilizationofMSmediumAcommonmediuminplanttissuecultureistheMSmedium.ThepreparationoftheMSmediumcomprisesthefollowingsteps・PreparationandpreservationofMSculturemediumcontainingnearly30kindsofnutritionalcomponentsofbaseliquor,inordertoavoid
2、everymediumpreparationshouldbecarriedoutontheweighingdozensofingredients,canbeculturedinthemediumofvariouscomponents,accordingtotheoriginalamountof20timesor200timesrespectivelyweighing,withconcentratedliquid,thisconcentratedsolutioniscalledculturebaseliquor・Inthisw
3、ay,eachuse,takethetotalamountof1/20(50mL)or1/200(5mL),dilutewithwater,intotheculturemedium・Thequantitiesofvarioussubstancesrequiredforthepreparationofthemotherliquidoftheculturemediumarelistedforuseinpreparation.Alargenumberofelements(motherliquorI)mg/LNH4N0333000K
4、N0338000CaC122H208800MgS047H207400KH2P043400Traceelements(MotherLiquorII)KI166H3B031240MnS044H204460ZnS047H201720Na2Mo042H2050CuS045H205CoC126H205Ironsalt(motherliquorIII)FeS047H205560Na2-EDTA2H207460Organicingredients(motherliquorIV)IVAInositol20000IVBNiacin100Pyr
5、idoxinehydrochloride(vitaminB6)100Thiaminehydrochloride(vitaminBl)100Glycine400Theamountofallthenutrientsmentionedabovewas200timesofconcentratedliquidexceptformotherliquor,whichwas20timesconcentratedliquid・Eachofthesemotherliquidsshallbeindividuallyseparatedinto1Ls
6、tocksolution.Thepreparationmethodofmotherliquor,liquorandliquorare:IIIVweighingseveralcomponentseachinthemotherliquorafterrespectivelywithdistilledwatertodissolveasmallamount,andthentheyareimmiscible,thefinalvolumeto1L.Thepreparationmethodis:LiquorIIIwillbecalledFe
7、S047H20andNa2-EDTA2H20respectivelyin450mLdistilledwater,heatingandstirringtodissolvethem,thenthetwosolutionsaremixed,andthepHwasadjustedto5.5andfinalvolumeto1L,storedinbrownglassbottle・Afterallthemotherliquorhasbeenprepared,theyarestoredinglassbottles,labeledwithmo
8、ther，snumbers,preparationtimes,datesandsoon.Theyarekeptintherefrigerator・MSmediumadding24-，twochlorophenoxyaceticacid(2,4-D)andNAA(NAA),6(6BA)and
