甘遂饮片的炮制工艺、质量标准与解毒机理.研究

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1、甘遂饮片的炮制机理研究fractionandsometoxiccompositionandeffectivecomponentsofkansui．ThroughHPLC—MS“analysis，wefounditcontainsalargenumberofditerpenoids，especiallyingenanediterpeneesters．WeextractedandseparatedthetoxicconstituentsfromrootsofEuphorbiakansuiinShanxibychromatographytech

2、nique．SixknowncompoundswereisolatedandidentifiedaSKansuiphorinC(1)，3-O-benzoyl-20-deoxyingenol(2)，3-D(2E4'Z-decadienoyl)一20一O-acetylingenol(3)，Euphol(4)，fl-sitosterol(5)，刀-hexadecanioeacid(6)．Finally,weanalyzedthechemicalconstituentsofethanolextractofkansuiandvinegar-prep

3、aringkansuibyHPLC—MSnanalysis．Theresultsshowedthatafterbeingprocessed，diterpenoidsdeclinedinawhole，especially3-O-benzoyl-20一deoxyingenoland5一O—benzoyl-20一deoxyingen01．Therefore，thepossibleprocessingmechanismofkansuiWaSthatditerpenoidsdeclinedinawholeafterbeingprocessedwit

4、llvinegar．BaSedonaboveresearchresults，selecting3-0-(21E，4'Z-decadienoyl)-20一。一acetylingenolasindicativeconstituent，thebestprocessingtechnologywassatisfied、)Iritllsomeconditionsbyorthogonaldesignasfollows：stirat260"Cfor9minbyadding30％vinegar．Theresultsshowedthatthetechniqu

5、eWasreaSonable，stableandreproducible．Simultaneous，wealsocarriedoutqualitystandardresearchincludingcharacteristics，microscopic，physico-chemicalidentification,TLC—identification,determinationofextracts，andquantitativedeterminationofradixkansuiandvinegar-preparingkansui．Inad

6、dition,wealsomadeanelementarystudyontheHPLCfingerprintofkansuiwhichWaSusedforcommodityanditsprocession．IntheestablishedHPLCfingerprintofkansuianditsprocessingone．17mutualpeaksweredemarcated．Boththeratioofadjustablereservingtimeofeachpeakandtherelativepeal【areacallbetakena

7、sthebaSisofkamuiquality,moreover,theyalsoprovideresearchdataforthequalitystandardoftheimportherbkansuianditsprocessingone．Themainfeatureandinnovationinthisthesisarepresented：1．UndertheguideofthetheoryoftraditionalChinesemedicine，wefoundtoxicfractionandactiveconstituentsby

8、chemicalseparationandcomparisonofpharmacologicalandtoxicologicalscreening，andwealsorevealsthepos