基于ssr标记构建白蜡种质资源分子身份证及其遗传多样性分析

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1、万方数据基于SSR标记构建白蜡种质资源分子身份证及其遗传多样性分析聚类分析结果不完全相符，来源地与聚类结果没有明显对应关系。关键词：白蜡；SSR；分子身份证；遗传多样性Ⅱ万方数据山东农业大学全日制硕士专业学位论文EstablishmentofMolecularIDforFraxinusBasedonSSRMarkersandAnalysisofGeneticDiversityAbstractFraxinusisakindofdicotyledonousplants，belongstooleace．ae(Oleceae),clovers

2、ubfamily(Olenideae)．Therearemorethan70kindsofFraxinusintheworldandabout34speciesinourcountry(201l，shaox-bin)．FraxinusisSakindofcommongreeningtreespeciesthathasoutstandingsalt-tolerantanddry-tolerant，whichiswidelydislzibutedinourcountry．Duetothevarietyofpewtergarmplasmre

3、solffges,theworkofidentificationofgermplasmresourcescausedtheallentionoftheresearchers．Bythelate1980s，withthecontinuousdevelopmentofmolecularmarkertechnologyandimprovementofmolecularlevelforFraxinusgermplasmreso蝴appraisallaidafoundation．TherepetitionrateofSSRmarkersislo

4、w,DNAdistributionismoreuniform,highstability,goodrepeatabilityandrichpolymorphism,codingregioninarabidopsisandotherplants，non-codingregionsarewidespread,forgermplasmresourcesprovidetheoreticalbasisforthepurityandauthenticityidentificationofvarieties．Inrecentyeats，somesc

5、holarshasmadecartainprogressandsuccessfullyinlitchiandblackwalnubYSSRteehniquet,andconstructedthemolecularIDofsoybean,banana,sugarcane，pearandpeachgermplasm陀sources．However,SSRmarkersontheashjusthavefewapplications．Thisstudyusingmicrosatellitemarkerstoforestryscienceres

6、earchinstituteofshandongprovinceshouguangteststationsave46oftheFraxinusasthematerial，whichllsetherelevantsoftwaretoanalyzed,mrou曲thestudyOnthemolecularlevel,tOexplorebuildingpewtergermplasmmolecularidentificationmethodprovidesasofidtheoreticalbasis，andlaythetheoreticalf

7、oundationforthefertherexaminationandapprovalDUStestingofnewvarietiesofwhitewax,molecularmarkerassistedbreedingandotherfields．Followinga∞themainresults：1,Constructionofmolecularidentification：46experimentalstationofFraxinusfromshouguanginshandongprovinceforestrysciencere

8、searchinstitutewerecollectedtobuildChineseFraxinusmolecularidentificationgermplasmresources．UsingSSRmarkerstod