萨克拉普奇尼亚菌(pochonia+suchlasporia)对南方根结线虫致病性的分析

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1、青岛农业大学硕士学位论文目录3．1．5Determinationmethodoftheparasiticrateofeggsandfemales3．2Resultsandanalysis3．2．1TheeffectofP．suchlasporiaonparasitismoffemales3．2．2TheeffectofP．suchlasporiaonparasitismofeggs4ThestudyofchitinaseproducedbyP．suchlasporia⋯⋯’4．1Materiaisandmethod

2、s⋯⋯··4．1．1Thestrainstested⋯⋯⋯··⋯⋯⋯⋯⋯⋯⋯⋯⋯27⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·27⋯⋯．．．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·274．1．2Thepreparationoftestreagent⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯28⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·30⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯30⋯⋯⋯⋯⋯⋯⋯．．30⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯304．1．3Comparisonofculturecondtionsforproducingchitinase-⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯314．1．4Com

3、parisonofdeterminationcondtionsofchitinase。4．1．5DeterminatechitinaseactivityindifferentN-containingculturemedium⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。324．1．6Thedeterminationofproteincontent⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·‘⋯⋯⋯⋯⋯⋯⋯⋯⋯344．1．7Theactive-stainingofchitinase⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

4、⋯。354．1．8Theproteinstaining⋯⋯⋯⋯⋯⋯⋯”4．2Resultsandanalysis⋯⋯⋯⋯⋯⋯⋯⋯⋯354．2．1Comparisonofculturecondtionsforproducingchitinase-·4．2．2Comparisonofdeterminationcondtionsofchitinase⋯⋯⋯⋯·⋯⋯⋯⋯⋯⋯⋯35⋯⋯⋯⋯⋯⋯⋯36⋯⋯⋯⋯⋯⋯⋯⋯⋯364．2．3TheresultsofchitinaseactiviWindifferentN-contain

5、ingculturemedium⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·‘374．2．4TheresultsofexdochitinaseactivityindifferentN-containingculturemedium⋯⋯⋯⋯⋯⋯⋯⋯¨384．2．5Theresultsofproteincontent4．1．6Theactive-stainingofchitinase。‘⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·⋯⋯⋯⋯⋯⋯⋯”⋯⋯⋯⋯⋯⋯⋯·39⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯405Theeffectofculturefiltratepr

6、oducedbyP．suchlasporiaonegghatchingofM．incognita⋯⋯⋯⋯⋯425．1Materialsandmethods⋯⋯⋯⋯·⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·425．1．1Thestrainstested⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯··⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·425．1．2Culturecondtionsforproducingchitinase⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯425．1．3Prep

7、arationofthesuspensionofroot—knotnematodeeggs⋯⋯⋯⋯⋯⋯¨⋯⋯⋯⋯⋯⋯⋯425．1．4Determinationmethodoftheinhibitionrateofegg-hatching⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·425．2Resultsandanalysis⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯¨425．2．1Theinhibitionrateofegg-hatching⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯‘⋯⋯⋯⋯

8、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯425．2．2ThemorphologicalchangesofeggshellofM．incognita⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”46青岛农业大学硕士学位论文目录6Conclusionanddiscussion7Prospect⋯⋯⋯⋯·7．1Improvingthecontroleffectofstability。