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ID:33202508
大小:5.45 MB
页数:34页
时间:2019-02-22
《开郁安神汤对慢性应激抑郁模型大鼠血清cort及海马camp表达影响研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、陕西中医学院硕士学位论文开郁安神汤对慢性应激抑郁模型大鼠血清CORT及海马cAMP表达影响研究姓名:蒋慧申请学位级别:硕士专业:中西医结合临床指导教师:林海201204中文摘要目的,根据祖国医学和现代医学对抑郁症病因、病机、治法的认识,通过观察开郁安神汤对慢性应激抑郁模型大鼠血清CORT及海马cAMP表达的影响,验证开郁安神汤在治疗抑郁症方面中的作用机理,并探讨开郁安神汤对抑郁症其机制可能与其参与调节HPA轴功能和细胞内信号传导有关。方法:采用慢性轻度不可预见性的应激抑郁模型加孤养。将实验大鼠分为六组:空白组、模型组、氟西汀组、开郁安神汤大、中、小剂量组,除空白组外,其余几组
2、均孤养,并给予轻度不可预见的应激刺激共2l天,分别给予相应实验药物灌胃,观察造模后大鼠的糖水偏嗜、敞箱实验得分、体重的变化、海马细胞形态,并利用利用放射免疫法检测血浆中CORT及海马cAMP表达情况。比较中药开郁安神汤大、中、小剂量组与盐酸氟西汀组及模型组和空白对照组血浆CORT含量和海马cAMP的含量。结果:模型组大鼠与空白组比较对蔗糖水的嗜好降低,体重增长速度缓慢,行为活动次数减少,敞箱活动得分降低;模型组大鼠与空白组比较血浆中CORT含量升高、海马cAMP的含量降低(尸<0.05)。开郁安神汤组与模型组比较降低模型大鼠血浆中CORT、升高海马cAMP的含量(尸<0.01
3、)。开郁安神汤组与盐酸氟西汀(对照组)比较均能提高抑郁症模型大鼠蔗糖水的偏嗜性,使体重增长速度增加,行为活动次数增多,敞箱活动得分增加及血浆中CORT含量降低、海马cAMP的含量升高开郁安神汤组优于对照组(尸4、鼠有抗抑郁作用。5.中药开郁安神汤具有疏肝理气、清心安神的功效,提示其抗抑郁作用可能与纠正HPA轴亢进,上调AC.cAMP.PKA信号通路有关。关键词l慢性应激抑郁模型I开郁安神汤l抑郁症I∞盯I海马cAMPTheeffeotoftheexperi:ontaIstudyofJieyugranuIoon00RTPISSlandcNiIPofhippooampaIoftheratinChronicstressdepressjon:odeIABST队CTObject:BasedontraditionalChineseandmodernmedicineforDepressionine5、tiology,pathogenesisandthreapy,theexperimentalstudyofJieyugranuleontheexpressioninthechronicstressdepressionmodelonCORTplasmaandcAMPofhippocampalabouttherat,SOastoverifythefurthereffectofJieyugranuleinthetreatmentofdepression.AndtoexplorethemechanismsthatJieyugranuleondepressionmayberelatdt6、oregulatetheHPAaxisandsignaltransmissionincell.Methods:Thedepressionmodelwasestablishedbyreceivedchronicunpredictablestressstimulus.Seventy—twofemaleratweredividedintosixgroups:controlgroup,modelgroup,fluoxetinegroup,high—doseJieyugranulegroup,middle—doseJieyugranulegroup,andlow—doseJieyugr7、anulegroup.After21daysofdifferentprocessing,allratswereweighed,measuredsucroseconsumptionandtakenopen—fieldtest.ThenwedeterminethecontentofCORTseparatedfrombloodplasma,anddeterminethecontentofcAMPofseparatedsideofrathippocampalusingradioimmunoassay.Tocom
4、鼠有抗抑郁作用。5.中药开郁安神汤具有疏肝理气、清心安神的功效,提示其抗抑郁作用可能与纠正HPA轴亢进,上调AC.cAMP.PKA信号通路有关。关键词l慢性应激抑郁模型I开郁安神汤l抑郁症I∞盯I海马cAMPTheeffeotoftheexperi:ontaIstudyofJieyugranuIoon00RTPISSlandcNiIPofhippooampaIoftheratinChronicstressdepressjon:odeIABST队CTObject:BasedontraditionalChineseandmodernmedicineforDepressionine
5、tiology,pathogenesisandthreapy,theexperimentalstudyofJieyugranuleontheexpressioninthechronicstressdepressionmodelonCORTplasmaandcAMPofhippocampalabouttherat,SOastoverifythefurthereffectofJieyugranuleinthetreatmentofdepression.AndtoexplorethemechanismsthatJieyugranuleondepressionmayberelatdt
6、oregulatetheHPAaxisandsignaltransmissionincell.Methods:Thedepressionmodelwasestablishedbyreceivedchronicunpredictablestressstimulus.Seventy—twofemaleratweredividedintosixgroups:controlgroup,modelgroup,fluoxetinegroup,high—doseJieyugranulegroup,middle—doseJieyugranulegroup,andlow—doseJieyugr
7、anulegroup.After21daysofdifferentprocessing,allratswereweighed,measuredsucroseconsumptionandtakenopen—fieldtest.ThenwedeterminethecontentofCORTseparatedfrombloodplasma,anddeterminethecontentofcAMPofseparatedsideofrathippocampalusingradioimmunoassay.Tocom
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