il-17基因多态性与汉族人群胃癌相关性研究

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1、授予单位代码10089学号或申请号13524HebeiMedicalUniversity硕士学位论文在职科学学位IL-17基因多态性与汉族人群胃癌相关性研究作者姓名：高伟导师：杨丽娟副教授专业：免疫学二级学院：河北医科大学基础医学院2015年03月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下,由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,第一署名为单位河北医科大学，试验材料、原始

2、数据、申报的专利等知识产权均归河北医科大学所有。否则,承担相应法律责任。研究生签名‘导师签章级学院领导盖章：赠年Jr月巧曰河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名导师签章:年上月巧曰目录中文摘要·············································································

3、1英文摘要·············································································4研究论文IL-17基因多态性与汉族人群胃癌相关性研究前言·············································································8材料与方法····································································9结果·····

4、········································································17附图·············································································21附表·············································································27讨论································

5、·············································31结论·············································································34参考文献·······································································35综述IL-17基因多态性与胃癌的关系········································39致谢···

6、················································································56个人简历·············································································57中文摘要IL-17基因多态性与汉族人群胃癌相关性研究摘要目的：通过采用PCR-RFLP技术及直接测序法对汉族胃癌人群IL-17基因已知的SNP位点rs2275913,rs3748067,rs4711998和rs

7、763780进行研究，比较各SNP位点等位基因在胃癌及正常对照人群中的分布，揭示IL-17基因的SNP位点与胃癌之间的相互关系。方法：本课题包括2013-2014年从河北省人民医院收集的386例经过内镜检查病理组织学确诊的胃癌患者及374例同期健康体检者，两组研究人群在年龄、性别构成上均相匹配。排除自身免疫及炎症性疾病，如系统性红斑狼疮、糖尿病、类风湿关节炎、炎症性肠病等，排除有胃癌家族史者，所有研究对象经血、尿、便常规和心电图检查均未发现异常。取4mlEDTA-K2抗凝血，提取基因组DNA,采用生物学软件Primer5.0设计引物，

8、并与GenBank进行比对。采用PCR-RFLP方法扩增目的基因片段，进行多态性分析。统计数据在SPSS19.0软件包中完成，各统计指标均以均数±标准差（mean±SD）表示。用基因计数法计算各基因型频率和等位2基因频率