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1、FoodAnal.Methods(2012)5:821–827DOI10.1007/s12161-011-9318-xDeterminationofFreeArginine,Glutamine,andβ-alanineinNutritionalProductsandDietarySupplementsJeffreyH.Baxter&PaulW.JohnsReceived:14August2011/Accepted:4October2011/Publishedonline:15October2011#SpringerScience+BusinessMedia,LLC20
2、11AbstractAmethodforthedeterminationoffreearginine,nutritionallydeficientproteinsource(e.g.,wheatorrice)inglutamine,andβ-alanineinnutritionalproductsanddietaryordertoimprovebiologicalutilization(Ottenheymandsupplementsisdescribed.TheaminoacidsarederivatizedJenneskens1970).Somenonessenti
3、alaminoacidshavewiththefluorescenttag9-fluorenylmethoxycarbonylfoundsimilaruse,inspecialapplicationswheretheirfree(FMOC),andthederivativesarethendeterminedbyform(a.k.a.crystalline)supplementationprovestherapeuticreversedphaseHPLC.Methodsuitabilitywasdefinedbyorperformance-enhancing.Thre
4、esuchnonessentialamino2experimentalassessmentsoflinearity(R>0.999),precisionacidsaretheconditionallyessentialandfunctionalamino(day-to-dayRSD≤1.0%),accuracy(spikerecoveries=acidsarginineandglutamine,andthenonproteinamino98.7%–101.8%,n=18),andselectivity(baselineresolutionacidβ-alanine(K
5、earnsetal.2001;FurstandStehle2004;fromtheothercommonaminoacids).ThemethodWu2009).Toanindividualinastateofstress,arginineprovidesforanaccurateandprecisequantificationofthemayconfermetabolic(e.g.,ammoniadetoxification),threeaminoacids,whenpresentatconcentrations>0.2%vascular(e.g.,nitricox
6、ideproduction),and/orimmunological(w/w)innutritionalproductsanddietarysupplements.(e.g.,lymphocyteproliferation)benefits(Heylandetal.2001;AssessmentsoffreeL-glutaminestabilityinthreedifferentAppleton2002;ZhouandMartindale2007;Robinsonetal.reconstitutedpowderproducts,asperformedbythemeth
7、od,2008)andglutaminemayconfergastrointestinal(e.g.,foundrecoveries>97%through24hatroomtemperature.decreasedintestinalpermeability),antioxidant(e.g.,glutathi-oneformationenhancement),antiinflammatory(e.g.,cyto-KeywordsArginine.Glutamine.β-Alanine.HPLCkineproductioneffect),immuno
