钛氧薄膜表面氨等离子体浸没离子注入以及生物化修饰

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1、西南交通大学硕士学位论文钛氧薄膜表面氨等离子体浸没离子注入以及生物化修饰姓名：梅馨申请学位级别：硕士专业：材料科学与工程指导教师：陈俊英20090501西南交通大学硕士研究生学位论文第1V页西南交通大学硕士研究生学位论文第V页AbstractInthiswork，Ti—OfilmsweresynthesizedbyUnbalanceMagnetronSputteringonsiliconsubstrates，andthentreatedbyplasmaimmersionionimplantati

2、on(Pro)usingammomagasesinordertointroduceaminogrouptoTi-0film’Ssurface．Thestructureandthechemicalcompositionofthesurfacewereanalysedbyatomicforcemicroscopy(AFM)andX-rayphotoelectronspectroscopy(XPS)．ThedensityofaminogrouponthesurfaceoftreatedTi-Ofilm

3、scanberationedbyAcidOrangeII．Afterthat，laminin(Ln)andfibronectinfin)wereimmobilizedtothesurfacebasedontheamidogroup．Themorphologyofendothelialcellswasobservedbyopticalmicroscopy．Intheend，humanumbilicalveinendothelialcells(HUVEC)primarycultureexperime

4、ntswereadoptedinvitrotoevaluatethecompatibilityoftheendothelialcellsonthesurfaceoftheuntreatedTi．Ofilms、PIII．treatedTi—OfilmsandtheTi-Ofilmsimmobilizedbybiomolecules．Themorphologyofendothelialcellswasobservedbyopticalmicroscopy．Thecontactan舀eofPill—t

5、reatedTi-0filmssurfacewaslowerthanthatoftheuntreatedTi—Ofilms．ItindicatesthatthewettabilityofPill—treatedTi—OfilmsisalittlehigherthantheuntreatedTi-0films．TheAcidOrangeIIandXPSresultsshowthatthefunctionalgroupofamidohasbeenintroducedtothesurfaceofTi-

6、Ofilmbyplasmaimmersionionimplantation．TheresultsofSEMandAFMsuggestthattheroughHessofthePill·-treatedTi··Ofilmssurfacehasbeenincreased．neresultofimmunofluorescenceindicatesthatlamininandfibronectinhasbeenimmobilizedtothePill。treatedTi．0filmsurface．End

7、othelialcellcultureexperimentsuggeststhat，theaminogroupsare西南交通大学硕士研究生学位论文第VI页helpfulfortheadhesionandproliferationofHUVEC．TheLamininandFibronectinCanfurtherenhanceHUVEC’Sadhesion，proliferation，andgrowth．Accordingtotheendothelialcellcultureexperiment

8、，comparedtothePill-treatedTi-OfillsimmobilizedwithLamininorFibronectinonly，thePIIItreatedTi-Ofilmsimmobilizedwithbothlamininandfibronectincanpromotetheadhesionandgrowthofendothelial．Alltheseresultsindicatedthattheionimplantationcanintroducefunctional