TREM2在非小细胞肺癌中的表达及意义

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1、浙江大学硕上学位论文摘要TREM．2在非小细胞肺癌中的表达及意义浙江大学医学院呼吸内科硕士生沈茜导师周建英中文摘要目的：检测TREM．2在非小细胞肺癌中的表达，探讨TREM。2与肿瘤发生发展的相关性，从而进一步探讨肿瘤形成的免疫机制，为肿瘤的免疫治疗寻找新的靶点。材料与方法：取非小细胞肺癌患者肺组织，RT-PCR法检测肺癌组织、癌旁组织和正常肺组织中TREM．2基因表达情况。分离非小细胞肺癌患者和正常人外周血单核细胞，RT-PCR法检测TREM．2的表达情况。分离C57BL／6小鼠的DC并培养至成熟DC。同时体外培养Lewis小鼠肺癌细胞株3LL。用RT-PCR

2、法分别检测未成熟DC、成熟DC与3LL中TREM．2的表达情况。此后将成熟DC与3LL体外共培养，RT-PCR法比较共培养前后TREM．2基因表达差异。结果：非小细胞肺癌患者肺癌组织和癌旁组织中TREM一2的表达高于正常肺组织，非小细胞肺癌患者与正常人外周血单核细胞中TREM．2的表达无明显差异。未成熟的小鼠树突状细胞表达TREM．2，而成熟树突状细胞和3LL细胞未检测到TREM．2的表达。将成熟DC和3LL细胞共培养后TREM．2的表达仍呈阴性结果。结论：TREM．2表达的上调与非小细胞肺癌的发生存在一定的相关性。提示TREM．2可能参与肿瘤发生的免疫应答过程

3、，为肿瘤的免疫治疗提供了新的理论依据。关键词：TREM．2，非小细胞肺癌，RT-PCR，树突状细胞，共培养II浙江大学硕上学位论文摘要T’pressionand"gnificancefTREM．2IIeexPresslOnandsignilicanceO1IVli。nI_non-small-celllungcarcinomaCandidate：QianShenSupervisor：JianyingZhouAbstract【Objectivei：ToinvestigatetheexpressionofTREM一2inthedevelopmentofnon-smal

4、l-celllungcarcinoma，inordertorevealtherelationshipbetweenTREM一2andtumorandfindthenewimmunologicaltargetingtreatmentofcancer．【Materialandmethods]：UsedRT-PCRtodetectthelevelofTREM-2mRNAinintratumoraltissue，peritumoraltissueandnormaltissueofnon-small—celllungcarcinomapatients．Peripheral

5、bloodmononuclearcells(PBMC)ofnon·small-celllungcarcinomapatientsandcontrolswereisolatedandwealsousedsemiquantitativePCRtodetecttheexpressionofTREM-2inmurine．Weisolateddendriticcells(DC)fromC57BL／6miceandculturedtomaturestate．Meanwhile，weculturedLewismurinelungcancercellline3LLinvitro

6、．TheexpressionofTREM一2ofimmatureDC，matureDCand3LLweredetectedbyRT-PCR．Thenweco·culturedmatureDCand3LLandcomparedthelevelofTREM一2mRNAtothatbeforeCO-culture．【Results]：WefoundthatexpressionofTREM-2mRNAofintratumoraltissue，peritumoraltissueweremoreobviousthannormaltissueofthenon-·small-·

7、celllungcarcinomapatients，whichtherewerenostatisticaldifferencesbetweenthetwoformers．TherewasnodifferenceinthelevelofTREM．2mRNAinPBMCbetweenthenon—small—celllungcarcinomapatientsandcontrols．WealsoobservedthatimmatureDCexpressedTREM-2butmatureDCand3LLdidnot．WeCO·culturedmatureDCand3LL

8、andstillgott