壳聚糖可被溶菌酶溶解

《壳聚糖可被溶菌酶溶解》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Availableonlineatwww.sciencedirect.comActaBiomaterialia5(2009)406–418www.elsevier.com/locate/actabiomatSynthesisandcharacterizationofelasticandmacroporouschitosan–gelatincryogelsfortissueengineeringaaba,*NeerajKathuria,AnujTripathi,KamalKKar,AshokKumaraDepa

2、rtmentofBiologicalSciencesandBioengineering,IndianInstituteofTechnologyKanpur,Kanpur208016,UttarPradesh,IndiabDepartmentofMechanicalEngineeringandMaterialsScienceProgramme,IndianInstituteofTechnologyKanpur,Kanpur208016,UttarPradesh,IndiaReceived8February200

3、8;receivedinrevisedform5July2008;accepted7July2008Availableonline25July2008AbstractElasticchitosan–gelatincryogelsofvaryingconcentrationofpolymerprecursorshavebeensynthesizedusingglutaraldehydeasacrosslinkingagent.Theoptimumco-polymerratioofchitosantogelati

4、nwasfoundtobe1:4atthetemperatureof12°Cforsynthesisofchitosan–gelatinhybridcryogels.chitosan–gelatincryogelssynthesizedwithlowviscositychitosanweremorphologicallybetterthanthoseformedwithmediumandhighviscositychitosan.Porediametersofchitosan–gelatincryogels

5、asmeasuredbyscanningelectronmicroscopy(SEM)wasintherangeof30–100lm.Whilemercuryporosimetryanalysisrevealedthemajorityofporesofthescaffoldlyingintherangeof30–50lm.Porosityofchitosan–gelatincryogelswasfoundtobegreaterthan90%usingArchimedes’sprinciple.Unconfined

6、compressiontestsshowedsignificantelasticityofchitosan–gelatincryogelsandmaintainedtheirphysicalintegrityevenaftercompressingthemupto80%oftheiroriginallength.Theelasticmodulusvariedintherangeof36–39kPa.Cyclicdeformationanalysisperformedbycompressionofchitosan

7、–gelatincryogelswithvaryingstrains(10,20and40%)showednocrackingoranysignificantdeformation.Thedegradationofchitosan–gelatincryogelswasfoundupto13.58±1.52%at37°Cwithin8weeksofincubationundersterileconditionsandthecryogelsswelledupto90%oftheircapacitywithintwo

8、min.Efficientcelladherence,proliferationandextra-cellularmatrix(ECM)secretionwasobservedbygrowingfibroblast(Cos-7)celllineonchitosan–gelatinhybridcryogelswhichindicatepotentialofthematerialfortissueengine