大鼠肝移植供肝热缺血时间与胆道损伤的关系

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1、万方数据大鼠肝移植供肝热缺血时间与胆道损伤的关系邰强何晓顺杨建安朱晓峰马毅巫林伟鞠卫强胡安斌王东平王国栋【摘要】目的探讨大鼠心源性死亡(DCD)供肝不同的热缺血时间与胆道缺血再灌注损伤的关系。方法采用无肝素化的大鼠DCD供体肝移植模型，按供肝的热缺血时间分为0min(W10)，10rain(w110)、15rain(W115)3组，每组36对大鼠。术后动态观察大鼠胆道病理改变及其并发症、肝功能指标，最后统计总体生存率。结果供肝热缺血小于10min时，术后胆道病理改变较轻且为可逆性改变，肝功能恢复较快；供

2、肝热缺血时间为15min时，术后胆道病理改变较重且为不可逆性改变，肝功能恢复延迟；3组胆道并发症的发生率差异有统计学意义(5．56％比8．33％比16．67％，P<0．05)。W10组和W110组的大鼠术后4周生存率差异无统计学意义(83．33％比77．78％，P>0．05)，而与WIl5组比较4周生存率的差异有统计学意义(83．33％比58．33％，77．78％比58．33％，P

3、移植；胆道病理；热缺血时间；缺血再灌注损伤Therelationshipbetweendifferentischemiatimeofnon-heartbeatinglivergraftandbileductischenda／reperfusioninratlivertransplantationTAlQiomg’。HEXiao·shun．YANG玉皿n-an。zHUXiao母ng，MAYi，WULin—wei．JUWei—qiang，HUAn—bin，WANGDongoping。碡ANGGuo—dong

4、．+DepartmentofTransplantationCenter，theFirstaffiZio胎dHospitalofSunYat-SenUnivers蚵，Guangzhou5l0080．ChinaCorrespondingauthor：HEXiao-shun．Email：gdtrc@163．com【Abstract】ObjectiveToelucidatetherelationshipbetweendifferentiscbemiatimeofDonationafterCardiacDeath

5、(DCD)livergraftandbileductischemia／reperfusioninratlivertransplantation．Meth-otisWechoseimprovedratlivertransplantationmoldfortheresearch．Theratsweredividedintothreegroupsbydifferentwarniischemiatime(WI)，suchas，0min(WIo，n=36)，10min(WIlO，n=36)，15min(W15，n

6、=36)．Weanalyzedtherecipientsurvivalrate，biliarycomplication，hverfunctionandpatholo-gYafteroperationinthethreegroups．ResultsThereweresignificantlydifferencesintheW10，WIIOandW115groupinbiliarycomplication(5．56％VS8．33％VS16．67％．P<0．05)．WhenischemiatimeWasin1

7、0min．thepathologychangeislittlerandthehverfunctionrecoverfasterthanWIl5group．nechangewagrecoverablein10minbutnotabove15min．TherewerenodifferencebetweenW10andW110groupinfoill'week’ssurvivalrate(83．33％VS77．78％，P>0．05)．Thereweresignificantdifferencesbetween

8、WIl5andthefirsttwogroupsinsurvivalrate(83．33％VS58．33％。77．78％VS58．33％，P<0．05)．ConclusionWhelltheischemiatimeofDCDlivergraftbeingabove15min．thebileductcellWasdanl-agedremarkablyandshowirreversiblechange．【Keywords】Livetranspl