大麦籽粒中主要黄酮类物质与四种农艺性状的QTL定位

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1、万方数据QTL与环境存在互作。关键词：大麦；DH群体；黄酮；QTL万方数据QTLMappingforFourAgronomicandMainFlavonoidContentsChun-LeiYu(BiochemistryandMolecularBiology)DirectedbyProf．Zong-YunFengAbstractBarleyisolleofthe硒tlrmajorfoodcropsinthewofld．itisatypical“fiveyuan”crops(graincrops÷foragecr

2、ops，economiCcrops。healthandenergycrop)，alsohasavarietyoffunctioncompound，suchasp·glucan，7-aminotutyricacid，flavonoids，ResistantstarchandSOon，catechin，myricetin，quercetin，kaempferolmainlyflavonoidswitheliminationofoxygenfreeradical，antioxidant，antibacterial，a

3、nti-inflammatory,antiallergic，antimutation，anti—tumor,protectthecardiovasculardiseaseandSOon．Theimportantagronomicandqualityofbarleyaregenerallycontrolledbymanygeneandinteraction，alsospecificexpressionindifferentenvironment，findcontrolcomplexquantitativetrai

4、tgenebyanysisandguidethebreedingthroughnearmolecularmarkers．Theresultsshowthat：1theoptimumofHPLCmethodandextractionprocessofthemajorflavonoidsinbarleythroughorthogonalregression，HPLCmethod，AgilentZorbaxEclipseXDB-C18(5}，tm，250mm．4．6mmidlcolumn；mobilephaseA-l

5、％glacialaceticaqueousjB-acetonitrile，gradientelutionprogram：0～10min，15％,-。30％B：10"--16min，30％～40％B；16～20min，40％～15％B：flowrate0．8ml／min，columntemperature30。C；detectionat280nm，injectionvolumeof10肚L．Theoptimumultrasoundconditionwasasfollows：methanolconcentratio

6、n，50％．soild-liquidratio，1：20，andultrasoundtime，60min．2Atotalof51QTLsweredetectedon7chromosomesinclude3ladditiveQTLsand20epistaticQTLs，10additiveQTLsand5epistaticQTLsrelatetOfourkindsofflavonoids；21additiveQTLsand15epistaticQTLsrelatetofouragronomictraits．QTL

7、distributiononthe7chromosomesatmost，2l，onthe1chromosomesatleast，only1whilethe2H，3Hj4H．5Hand6Hchromosomeswere14，8，9and2．Inthisstudy，Sevenclustersinvolvingmorethanonetraitweredetected．whichwerelocalizedonchromosomes2H，TTT万方数据3H，4Hand7H，onthe7chromosomesatmost，

8、3．onthe3chromosomesatleast，only2，whilethe2Hand4Hchromosomeswere1，thisexperimentconsideringQTLandinvironmentinteraction．wedetected1additiveQTLand6epistaticQTLwerewithsignificantenvironmentaleffec