大鼠肾上腺细胞体外培养观察与鉴定

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1、AbstractABSTRACTObiective：Toconducttheprimarilycultureofratadrenocorticalcells，andtoobservethecharacteristicsofgrowthandsecretionincellcurtureindifferentphasewhichwillhelpofferexperimentevidenceforsearchingthemostsuitabletimeofadrenalcelltransplantation．Me

2、thods：AdrenalglandswereremovedfromtwoweekoldWistarratsundersterileconditions，thenthecoaexwasseparatedandshredded．ThecellswereprimarilyculturedinDMEMmediumcontaining、析t1120％fetalbovineserunl．Thegrowthprocessandmorphologicalfeaturesofthecellswereobserveddyna

3、micallyundertheinvertedphasecontrastmicroscope，meanwhile，thesurvivalrateofthecellsweredetectedbystaining谢tlltrypanblue．Atdifferentculturetimes，theconcentrationofcorticosteronewasdeterminedbyenzymeimmunoassay，corticosteroidandaldosteronebyradioimmunoassay．R

4、esult：Afternormaladrenocorticalcellswereprimarilycultured48hours，cellswereattachedtothebottomofculturedishesandvolumebegantoaugment，mostofwhichwereroundorpolygonalshaped，andcellbodyWasbigandcytoplasmWaslucency．Furthermore，therearemanygranaintracytoplasmand

5、it’Sbulkiscomparativelyregular．cellsgrewprosperitily,cellquantityincreased，whichbecameconfluentandthesurvivalrateofthecellsreachedto95．94％fourdaysafterplming．Withelongationofculturingtime，cellsbecamefatandfusiformshapegradually，granaintracytoplasmgrewdownw

6、ardsbydegrees．After2weeks，cellsgraduallybecameshrunken,brokenandsenesced．Inadrenocorticaleellcultureinvitro，corticosteroidmeasurementsshowedthatcorticosteroidconcentrationWasincreasingwiththetimeextending，incontrasttograduallydecreasedandthenenteredintoapl

7、ateauphaseafterfourdays．Theconcentrationsofcortieosteroneandaldosteronewereincreasinggraduallyinthe2th，4th，6thor7thday．Itreachedtoapeal【inthe7thday，andthendegradedinthelOthday．Comparedtothepeal(，therewasasignificantdifference(P<0．01)，butIIIAbstract一———————

8、———_——————————————————————_———————_————————————————一corticosteroidwasstepedupalittle．ConeIusion：Normalrat’Sadrenocorticalcellsculturedprimarilyinvitrohavethefunctionofsecretingadrenocorticalhormone．Glucocorti