放线菌分离方法的分析

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1、STUDYONISOLATIONⅣ庄!THODSOFACTINOMYCETESABSTRACTActiIlomyCetesisak砌ofmicrobetllatclo∞ly托lated诵tllhllm趾life．Ith勰伊eatutilizationvmmiIli11d删时，andh勰madegreatcoI蚵butionforhum锄．OIlly10％~20％aCtillomycetcscouldbeisol砷edatpre∞m．Ne砌y80％actiJlornycetescouldn’tbeisolatedby位ldi

2、tionalisolationm“hods．Theexploration趾ldutilizationofm岫wn孤ltinomycetcsresourceis锄u玛emproblemof∞illIlicrobiology．Thee髓ctofnlicrowave—pren．ea：cn把鸣∞ilparticlesizeand∞ilsuspenSiondisperS乏mton廿leisol撕onof觚tinomycetesisreponediIlt陆s代search．TheresuhsaIl}easfbnow：1．AdaptiV

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5、4minutes，tllenewlyidemifiedactinoIIlycetesspIecies，madeup62．5％-85．7％and66．7％-83．3％ofallthe∞t劬rnycetesS】pecies∞tlleGAandHAculturemediur玛他spectiVely．MicrowaVeirI硼iationcouldexert∞marl【a_blei11nuenceontllenumbe璐of孤ItiIIlicrobial∞tinomycetes鼬吼iI塔，w11iletllemicmwaVeirr

6、adiationsl嬲ted6，9锄d15mimnes，mepercemagesof锄timicrobial趾tiIlomycetes蛐阻iI塔on廿Ietv旧叫tu坞medi啪sm粥勰edby66．7％，66．7％and83．3％iIlc伽叩ariS0n谢也those0ntlleco砷rolmedi眦，respectiVely．2．Withnle血crowaveimdiationdl删io璐iIlcre嬲ed，廿ledryS0ilpre∞ntedtotal∞tiIlomycetes趾ds所印，D明肜缈qu觚tit)rma

7、ttendedt0硒tiallyinCrea∞觚dttl吼decre勰e，theq啪ntit)rof脚纪阳历D枷嘎印朋iIlc陀嬲edsi班fic锄tlyiIlcomparison嘶mⅡlecontrolmedi啪．Wmletllemicr0、相veimdiationlaSted、柝thiIl3minlItes，nletotalactirlom”，etes，哗唧钟and册衙D历D撇跏D阳q咖tities咖edby71．3％，102．9％and212．5％incomparison、^，immo∞onnlecontrolmed

8、i眦．Mic∞wavem们iationexertcd陀markable面【fluences0ntIlemmlb盯ofm他actinomycetesgenera，aIldthepercentof锄痂[Ilicrobial∽tiIloInycetesiIlcre硒edsigIlificantlyillcom