低氧对人外周血T淋巴细胞生存活性的影响与其机制分析

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1、低氧对人外周血T淋巴细胞牛存活性的影响及其机制研究StudyoftheeffectsandmechanismsofhypoxiaonthesurvivalactivityofhumanperipheralbloodTlymphocytessurvivalactivityolhumanperipheralD1lympnocytesAbstractMajor：ImmunologyPostgraduate：ZhangYahSupervisor：Prof．QuXunImmunesystemshavetheabihtyofdefence，surveillanceandimmunologicalhome

2、ostasis，throughrecognizeselfandun—self,whichisessentialforthebody．Tcells，themajorcomponentofthelymphocytesandtheimportantimmuneeffectorcells，mediatethespecificimmuneresponse．LocaltissuemicroenvironmentinfluencethefunctionofTlymphocytes，includetheoxygenconcentrationandthesecreteofcytokineandSOon．The

3、studiesbasedonthetumor-bearedanimalmodelandthecancerpatientshavesuggestedthatthefunctionoftheimmunecellswereseriouslyinhibitedatthetumorlocation．Oneofthedominantfeaturesofthetumorlocalmicroenvironmentisserioushypoxia，whichcouldimpactonthetypeandresultoftheimmuneresponse，andparticipateinthedevelopme

4、ntandtulTloveroftheinflammationandtumorcharacterizedbythelocalhypoxiamicroenvironment，butfewerreportswererefertothechangesoffunctionsandphenotypesandtheirmechanisms．Inrecentyears，itisreportedthattheexpressionofCCR7onthesurfaceofTlymphocytesiscorrelatedwiththeirsurvivalactivity．Ithasbeenconfirmedtha

5、ttheaccelerationofapoptosisisrelevantonthedown-regulatedexpressionofCCR7onTlympocytes，butthemechanismisneverknown．Wemadethestudyaboutthereasonandmechanismoftheweakedimmuneresponseonthetumorenvironmentbasedonthehypoxia-drivedsignalpathwaystothesurvivalactivityandCCR7expressiononTlymphocytes．Ithassig

6、nificantmeaningsontheunderstandingoftheinnateandadoptiveimmunityanddevelopingnewlyandmoreselectively3山东省医学科学院硕士学位论文anti-inflammatoryandanti-tumortherapies．1．ObjectiveInvitromimicthetumorhypoxiamicroenvironmenttoexamtheimpactofhypoxiaonthesurvivalactivityofTlymphocytesanditsmechanism,andtorevealthem

7、olecularbasisoftheimmunetoleranceandimmuneescapewiththepurposestoprovidethebasaltheoryonthetumorvaccinedevelopmentandimmunology—basedcancertreatments．2．Methods2．1IsolationandcultureofhumanperipheralbloodTly