资源描述：
《钙离子对盐胁迫小麦幼苗氮代谢的影响_王志强》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、第29卷第8期生态学报Vo.l29,No.82009年8月ACTAECOLOGICASINICAAug.,2009钙离子对盐胁迫小麦幼苗氮代谢的影响*王志强,王春丽,王同朝,林同保(河南农业大学农学院/作物生长发育调控省部共建教育部重点实验室,郑州450002)2+摘要:为探讨增强小麦抗盐能力的调控途径,以普通小麦豫麦34为材料,研究了Ca对盐胁迫下小麦幼苗氮代谢及生长的影响。采用全营养液培养小麦幼苗至第一片叶完全展开,更换无钙营养液,并开始不同处理。处理分别为低盐胁迫(150-1-12+-1
2、-12+mmolLNaCl)、低盐胁迫+4mmolLCa、高盐胁迫(300mmolLNaCl)、高盐胁迫+4mmolLCa,以无NaCl胁迫的2+小麦为对照。5d后取样,测定了氮同化酶活性、代谢物含量、积累量及幼苗生长状况。结果表明,Ca明显缓解了低盐胁迫对2+小麦幼苗的生长抑制,表现在鲜重、叶绿素及可溶性蛋白含量的增加,而对高盐胁迫下小麦幼苗的生长无明显改善效果;Ca改善了低盐胁迫下小麦幼苗的氮营养状况,表现在氮积累量的增加,这一效应主要是通过硝酸还原酶(NR)、谷氨酰胺合成酶2+(G
3、S)以及异柠檬酸脱氢酶(NADP-ICDH)活性的增强而实现的。Ca未能改善高盐胁迫下小麦幼苗氮营养状况的主要限制因子在于NADP-ICDH活性未明显增加。关键词:小麦;盐胁迫;钙离子;氮素代谢文章编号:1000-0933(2009)08-4339-07中图分类号:Q945.79文献标识码:A2+EffectsofCaonnitrogenmetabolisminwheatseedlingsexposedtosaltstress*WANGZh-iQiang,WANGChun-L,iWANGTo
4、ng-Chao,LINTong-BaoAgronomyCollegeofHenanAgriculturalUniversity,KeyLaboratoryofMOEforRegulatingandControllingCropGrowthandDevelopment,Zhengzhou450002,ChinaActaEcologicaSinica,2009,29(8):4339~4345.Abstract:Toseekwaysformiprovingsalt-toleranceofwheatpl
5、ants,expermientswereconductedtoexaminetheameliorative2+effectsofCaonnitrogenmetabolismundersaltstressusinganordinarywheatcultivar(TriticumaestivumL.cv.Yumai34)asthemateria.lThewheatseedlingswereculturedinfullnutrientsolution.Thefirstleafbeingfullyexp
6、anded,-1differenttreatmentswereinitiatedbyculturingtheplantsinthenutrientsolutionwithoutcalcium.NaCl(150mmolLand-12+-1-1-1-1300mmolL)andcombinationsofNaCl+Ca(150mmolL+4mmolLand300mmolL+4mmolL)wereappliedtothegrowingplantsusingnosalt-stressedpla
7、ntsascontro.lAfter5daysoftreatments,theactivitiesofnitrogenassmiilationenzymes,theamountofnitrogenassmiilationandthegrowthstatusofwheatseedlingswere2+determined.TheresultsshowedthatCasignificantlyalleviatedthedepressed-growthofwheatseedlingsunderlows
8、alt2+stress,manifestingtheenhancedfreshweight,chlorophyllandsolubleproteincontents;whileCadidnotshowobvious2+ameliorativeeffectsunderhighsaltstress.Capromotedthenitrogenassmiilationandmiprovedthestatusofnitrogennutritioninwheatplantsexposedtolowsalts
