资源描述：
《Efficient Generation of Gene-Modified Pigs》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Humu-2015-0327MethodsEfficientgenerationofgene-modifiedpigsharboringpreciseorthologoushumanmutationviaCRISPR/Cas9-inducedhomology-directedrepairinzygotes†#†#‡†††XiaoyangZhou,LuluWang,YinanDu,FeiXie,LiangLi,YuLiu,†††‡ChuanhongLiu,ShiqiangWang,ShibingZhang,XingxuHuang,Yong†*†*W
2、ang&HongWei†DepartmentofLaboratoryAnimalScience,CollegeofBasicMedicalSciences,ThirdMilitaryMedicalUniversity,Chongqing400038,China.‡SchoolofLifeScienceandTechnology,ShanghaiTechUniversity,100HaikeRd.,PudongNewArea,Shanghai201210,China.#Theseauthorscontributedequallytothiswork
3、.*Correspondenceauthors:HongWeiGaoTanyanStreet,Shapingbadistrict,Chongqing400038,China;Tel:86-23-68752261;E-mail:weihong63528@163.comThisarticlehasbeenacceptedforpublicationandundergonefullpeerreviewbuthasnotbeenthroughthecopyediting,typesetting,paginationandproofreadingproce
4、ss,whichmayleadtodifferencesbetweenthisversionandtheVersionofRecord.Pleasecitethisarticleasdoi:10.1002/humu.22913.Thisarticleisprotectedbycopyright.Allrightsreserved.1YongWangGaoTanyanStreet,Shapingbadistrict,Chongqing400038,China;Tel:86-23-68752262;E-mail:yongw7528@hotmail.c
5、omFunding:Y.W.wassupportedbygrantsfromNationalNaturalScienceFoundationofChina(31271330,31171280),National“863”ProjectofChina(2014AA021602)andChonqingMunicipalScientificProgramforBasicandFrontierAreas(cstc2013jcyjC80001).H.W.wassupportedbygrantsfromNationalNaturalScienceFounda
6、tionofChina(81173126)andNational“973”ProjectofChina(2011CBA01006).Y.L.wassupportedbygrantsfromNationalScience&TechnologySupportProgram(2011BAI15BO2)andNationalMajorScientificProjectsSpecificallyforR&DofTransgenicOrganisms(2014ZX08009-003-006).Thisarticleisprotectedbycopyright
7、.Allrightsreserved.2AbstractPrecisegeneticmutationofmodelanimalsishighlyvaluableforfunctionalinvestigationofhumanmutations.CRISPR/Cas9-inducedhomology-directedrepair(HDR)isusuallyusedforprecisegeneticmutation,beinglimitedbytherelativelylowefficiencycomparedwiththatofnon-homol
8、ogousendjoining(NHEJ).AlthoughinhibitionofNHEJwasshowntoenhanceHDR-d
