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1、AngewandteChemieDOI:10.1002/anie.201407021BiosensorsVeryImportantPaperTranslatingBacterialDetectionbyDNAzymesintoaLitmusTest**KhaTram,PushpinderKanda,BrunoJ.Salena,ShuangyanHuan,*andYingfuLi*Abstract:MicrobialpathogensposeseriousthreatstopublicUreasecatalyzesthehydrolysisofureaintocarbon[4–6]healt
2、handsafety,andresultsinmillionsofillnessesanddeathsdioxideandammonia.Thehydrolyticreactionraisestheaswellashugeeconomiclossesannually.LaboriousandpHvalueofthesolution.Ureaseishighlyefficient,speeding14expensivepathogentestsoftenrepresentasignificanthindranceupthehydrolysisofureabyabout10times.Urea
3、seisalsotoimplementingeffectivefront-linepreventativecare,partic-astableenzymeandvariousformsofureasesarecommer-[7,8]ularlyinresource-limitedregions.Thus,thereisasignificantciallyavailable.Thus,wepostulatethatureaseincombi-needtodeveloplow-costandeasy-to-usemethodsforpathogennationwithlitmusdyes(o
4、rpHpapers)aresuitedforthedetection.Herein,wepresentasimpleandinexpensivelitmusdevelopmentofcolorimetricbiosensors.Todoso,astrategyistestforbacterialdetection.Themethodtakesadvantageofrequiredtocoupleamolecularrecognitioneventtotheabacteria-specificRNA-cleavingDNAzymeprobeastheactivityofurease.mole
5、cularrecognitionelementandtheabilityofureasetoFunctionalnucleicacids,particularlyDNAaptamersandhydrolyzeureaandelevatethepHvalueofthetestsolution.Byaptazymes(aptamer-regulatedDNAzymes),havebeencouplingureasetotheDNAzymeonmagneticbeads,theshowntobeexcellentmolecularrecognitionelementsdetectionofbac
6、teriaistranslatedintoapHincrease,whichcanbecausetheyofferhighaffinityandspecificityfortheir[9–18]bereadilydetectedusingalitmusdyeorpHpaper.Thecognatetargets,andtheyarestableandcost-effective.simplicity,lowcost,andbroadadaptabilitymakethislitmusManyaptazymeshavebeenengineeredusingRNA-cleavingtestat
7、tractiveforfieldapplications,particularlyinthedevel-DNAzymes,wheretargetbindingtriggersthecleavageofan[18]opingworld.RNA-containingsubstrate.Someoftheseaptazymeshavebeenlinkedtosignal-generationmodulestoproducefl
