Association of a D-Alanyl-D-Alanine Carboxypeptidase Gene with the Formation of Aberrantly Shaped Cells during the Induction of Viable but Nonculturable Vibrio parahaemolyticus英文

《Association of a D-Alanyl-D-Alanine Carboxypeptidase Gene with the Formation of Aberrantly Shaped Cells during the Induction of Viable but Nonculturable Vibrio parahaemolyticus英文》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、AssociationofaD-Alanyl-D-AlanineCarboxypeptidaseGenewiththeFormationofAberrantlyShapedCellsduringtheInductionofViablebutNonculturableVibrioparahaemolyticusabaWei-chengHung,Wann-NengJane,Hin-chungWongaDepartmentofMicrobiology,SoochowUniversity,Taipei,Taiwan,Republicof

2、China;InstituteofPlantandMicrobialBiology,AcademiaSinica,Taipei,Taiwan,RepublicofChinabVibrioparahaemolyticusisahalophilicGram-negativebacteriumthatcauseshumangastroenteritis.Whentheviablebutnon-culturable(VBNC)stateofthisbacteriumwasinducedbyincubationat4°CinMoritam

3、inimalsaltsolutioncontaining0.5%NaCl,therod-shapedcellsbecamecoccoid,andvariousaberrantlyshapedintermediateswereformedintheinitialstage.ThisDownloadedfromstudyexaminedthefactorsthatinfluencetheformationoftheseaberrantlyshapedcells.Theproportionofaberrantlyshapedcellsw

4、asnotaffectedinamediumcontainingD-cycloserine(50g/ml)butwaslowerinamediumcontainingcephalosporinC(10g/ml)thaninthecontrolmediumwithoutantibiotics.Theproportionofaberrantlyshapedcellswashigherinaculturemediumthatcontained0.5%NaClthaninculturemediacontaining1.0or1.5%

5、NaCl.Theexpressionof15of17selectedgenesassociatedwithcellwallsynthesiswasenhanced,andtheexpressionofVP2468(dacB),whichencodesD-alanyl-D-alaninecar-boxypeptidase,wasenhancedthemost.TheproportionofaberrantlyshapedcellswassignificantlylowerinthedacBmutantstrainthaninthep

6、arentstrain,buttheproportionwasrestoredinthepresenceofthecomplementarydacBgene.ThisstudysuggeststhatdisturbanceofthedynamicsofcellwallsynthesisbyenhancedexpressionoftheVP2468geneisassociatedwiththehttp://aem.asm.org/formationofaberrantlyshapedcellsintheinitialstageof

7、inductionofVBNCV.parahaemolyticuscellsunderspecificconditions.VibrioparahaemolyticusisahalophilicGram-negativebacte-shape,andarabbitear-likeshape(15).Sincetheshapeofabacterialriumthatcausesfood-bornegastroenteritis(1).Mostclinicalcellisdeterminedbythefunctionsofthecel

8、lwall-synthesizingen-isolatesarehemolyticonWagatsumaagar(Kanagawaphenome-zymes/penicillin-bindingproteins(PBPs)andcytoskeletonprote