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1、微生物学通报MicrobiologyChinatongbao@jm.ac.cnFEB20.2011,38(2):2141220©2011byInstituteofMicrobiology,CAS番茄青枯病拮抗菌筛选鉴定及其发酵条件初探黄明媛I顾文杰2张发宝2徐培智2杨少海2王立群'76.72%価基金项目：nM•(No.2009A020101003)通讯作者:Tel:86-451-55190519;IHlwangliqun2@163.com收稿日期:2010-07-22;接受日期:2010-11-23解开治2(1.nK&b卩WgiV150030)
2、(2.n^5e3jnoo510640)摘要：从健康番茄根系采样，筛选出4株对番茄青枯病有较强拮抗作用的菌株，在NA培养基丄抑菌圈直径＞9nun。其中拮抗菌株YB6抑菌活性最强且拮抗效果稳定，通过形态学观察及部分生理生化特征测定，初步确定为节杆菌属。通过单因索试验进行了发酵条件初步硏究，得到适宜的发酵条件为：发酵时间3d,培养温度30°C,初始pH値9.0,接种量3%,转速100r/min,碳源蔗糖，氮源酵母浸膏。通过初步优化后拮抗菌株抑菌活性明显增强，最终对青枯病菌SST-Y和G2M1.70抑菌圈直径与NB培养基相比增加了76.72%和81.1
3、4%,差异显著。关键词：番茄青枯病，拮抗菌株，生理生化，发酵条件，抑菌活性IdentificationandfermentationofantagonisticbacteriumagainstRalstoniasolanacearumHUANGMing-Yuan1GUWen-Jie2ZHANGFa-Bao2XUPei-Zhi2YANGShao-Hai2WANGLi-Qun1*XIEKai-Zhi2(1.CollegeoflifeScience,NortheastAgriculturalUniversity.Harbin,Heilongjiang
4、150030,China)(2.SoilandFertilizerInstitute^GuangdongAcademyofAgriculturalScience,Guangzhou,Guangdong510640,China)Abstract:TheobjectiveofthisstudywastoscreenandidentifyantagonisticbacteriaagainstRalstoniasolanacearumfromtomatorhizospheresoil.Fourstrains(YB1,YB6,YB22,YB70)show
5、edstrongan tagonisticactivity,withdiametersofinhibitionzoneabove9mm.ThestrainYB6，identifiedasAr-throbcictersp.accordingtoitsmorphological,physiologicalandbiochemicalcharacteristics,presentedthebestandmoststableeffectagainstR.solanacearum・ThefermentationconditionsofthestrainY
6、B6were30°C,pH9.0,inoculum3%,shakingat100r/min,sucroseascarbonsource,andyeastcondensateasnitrogensource・Undertheoptimizedfermentationconditions,diametersofinhibitionzoneproducedbythestrainYB6againstR・solanacearumstrainsSST-YandG2M1.70increasedbyhtipJ'/wwWaCnki.net91994-2011Ch
7、inaAcademicJournalElectronicPublishingHouse.Allrightsreserved.21581.14%,respectively.Keywords:Ralstoniasolanacearum,Antagonisticbacteria,Physiologicalandbiochemicalcharacteristics,Fermentationcondition,AntibacterialactivityyvIA(Ralstoniaso lanacearum)Qi□t)qc=Tc=r<_LdOSL50、VU
8、L21bSIAaSIAU
9、AA282d心IA3河，彳450VaseosoVIAP〜O16[12]IA「bc:>aSFTIABA3>-^q^r計
