一株辣椒尖孢炭疽病菌拮抗菌株的分离鉴定与发酵条件优化

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1、一株辣椒尖抱炭疽病菌拮抗菌株的分离鉴定与发酵条件优化植物病理学报ACTAPHYTOPATHOLOGICASINICA42⑷:418-424(2012)一株辣椒尖砲炭疽病菌拮抗菌株的分离鉴定与发酵条件优化(1湖南农业大学生物安全科学技术学院'长沙410128;2植物病虫害生物学与防控湖南省重点实验室，长沙410128)朱宏建12欧阳小燕12周倩12高必达1,2*摘耍:为了探寻辣椒尖抱炭疽病的生防药剂，从而控制该病在辣椒生产上的扩散。以辣椒尖抱炭疽病菌(Colletotrichumacutata)为指示菌，从浏阳河风光带土样中分离筛选出6株拮抗放线菌株，其中菌株ND045对指示菌的拮抗效果最好

2、，菌丝生长抑制率高达71.6%O经形态观察、培养性状、生理生化鉴定,并结合16SrDNA序列分析，将ND045菌株鉴定为吸水链霉菌(Streptomyceshygroscopicus)。抗菌谱研究表明，该菌株对稻瘟病菌(Magnaportheoryzae)>辣椒疫霉病菌(Phytophtho-racapsici)>黄瓜枯萎病菌(Fusariumomysporum)和辣椒白绢病菌(Sclerotiumrolfsii)都具有较强的拮抗作用，抑菌率达40.58%~72.00%。通过单因子变量法和均匀设计法对菌株ND045进行发酵条件研究，结果表明,其最佳的发酵条件为:15g大米粉、5g大豆粉、0

3、.5gMgSO4>0.01gFeS04•7H2O>1.5gNaCK水1000mL、30°C、pH8。经优化后，菌丝牛长抑制率最高达到82.61%,比优化前提高了11.01%o该研究结果为辣椒尖泡炭疽病的生物防治提供科学依据。关键词:放线菌;辣椒尖抱炭疽病菌;吸水链霉菌;发酵条件IsolationjdentificationandoptimizingfermentationconditionsofanantagonisticstrainagainstColletotrichumacutataZHUHong・jianl2OUYANGXiao・yanl2ZHOUqianl2GAOBi・dal,2

4、(ICollegeofBio-SafetyScienceandTechnology,Changsha410128,China;2HunanProvincialKeyLaborato-ryforBiologyandControlofPlantDiseasesandlnsectPests’Changsha410128,China)Abstract:!nordertoexplorebiocontrolagentstocontrolpepperanthracnosediseasespreadinpepperproduc-tion.Sixantagonisticactinomycetesstrain

5、swereisolatedfromthecultivatedsoilsamplesofLiuyangRiverSce-nicBelt,amongwhichND045strainwasexhibitedthehighestantagonisticactivityagainstCollectotrichumacutataandshowedtheinhibitionrateof71.6%.TheND045wasidentifiedasStreptomyceshygroscopicusaccordingtothemorphology,culturecharacteristics,physiolog

6、icalandbiochemicaltestsandl6SrDNAanaly・sis.FurtherantimicrobialspectrumofND045wastestedagainstMagnaporthegrisea,Phytophthoracapsici,FusariumomysporumandSclerotiumrolfsiiandshowedtheinhibitionratebetween40.58%and72.00%・TheoptionalfermentationconditionsforantibacterialsubstanceproductionofNDO45werer

7、iceflourl5.0g,soy-beanflourlOg,MgS040.5g/FeS04-7H200.01g/NaCll.Sg^waterlOOOmLJermentationtemperaturemycelialgrowthwasupto82・61%,increasedforll.01%morethanbefore.Theresultsprovidescientificevidencetopepperanthracn