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1、Vet.Pathol.24564-567(1987)BRIEFCOMMUNICATIONSCalfhoodCoronavirusEnterocolitis:ACluetotheEtiologyofWinterDysenteryH.J.VANQUININGEN,L.H.KHAIRALLAH,V.G.SASSEVILLE,M.S.WYAND,ANDJ.E.POSTRecentlywepublishedareviewofwinterdysenteryanddestructionofepithelialcellsproducedtw
2、ocharacteristicele-documentedfindingsinspontaneousandexperimentaldis-ments:amoth-eatenappearanceofthemucosaatlowmag-easeinNewEnglanddairycattle.’OThehistologicfeaturesofnificationandstrikingnuclearpyknosisandcytoplasmichya-winterdysenterywerecharacterizedforthefirs
3、ttime.Ale-linedropletsathighmagnification(Fig.1).Acowthatdiedsionofthecolon,particularlythespiralcolon,wasrecog-hadwidespreadchangesofthistypeaswellasreparativenized.Itconsistedofpyknosis,karyorrhexis,granularde-dilatedcrypts.Thesefeatureswererecognizedasuniqueandg
4、eneration,hydropicdegeneration,andhyalinedropletsuggestiveof“virus-inducedenterocolitis.”degenerationofcryptepithelialcells.ThisscatteredfocalWhileourworkwasinprogress,investigatorsfromJapan9Fig.1.Coloniccryptfromspontaneouscaseofwinterdysentery.Epithelialcellswith
5、variousdegenerativeandnecroticchanges.Withincryptlumen,dyingcellscontainkaryorrhecticnucleiandhyalinedroplets.HE.Fig.2.Histologicsectionofspiralcolonfromcalfwithcoronavirusenterocolitis.Extensivecryptepithelialcellpyknosis,karyorrhexis,lysis,andregenerativehyperpla
6、siaidenticaltothatoccumnginwinterdysentery.Lossofcryptsleukocytosis,tatteredsurfaceepithelium,andgranulocyticexudateatthesurface.HE.564BriefCommunications565Fig.3.Negativelystainedcoronavirusparticlesinintestinalcontentsfromaffectedcalf.Sizefrom95-115nm,130-150nmwi
7、thpeplomeres.Bar=116nm.Fig.4.Numerouscoronavirusparticlesand“virusfactories”withindegenerateepithelialcellfromdamagedcolonicmucosaofcalf.Formalin-fixedtissue,virusparticles60nmindiameter.Bar=200nm.Fig.5.Numerouscoronavirusparticlesand“virusfactories”withinadegenera
8、teepithelialcellfromdamagedcolonicmucosaofcowwithwinterdysentery.Bar=200nm.566BriefCommunications:;-4,Fig.6.Extensiveimmunoperoxidasereactivitywi
