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1、J.gen.Virol.(1982),60,385-390.PrintedinGreatBritain385Keywords:HCVOC43/NCDCV/membrane-associatedantigens~fluorescentantibodytechniqueReactivityofHumanCoronavirusOC43andNeonatalCalfDiarrhoeaCoronavirusMembrane-assoeiatedAntigens(Accepted22January1982)SUMMARYHumanembr
2、yoniclungfibroblastculturesandVerocellculturesinfectedwithcellculture-adaptedstrainsofhumancoronavirus(HCV)OC43orneonatalcalfdiarrhoeacoronavirus(NCDCV)wereshowntopossesshighlycross-reactivemembrane-associatedantigens(MAA)bytheindirectfluorescentantibodytechnique(IF
3、AMA).MAAappeared3hpost-infection,concurrentlywiththeappearanceofcytoplasmicantigens.Electronmicroscopicobservationsofcellculturesinfectedwitheithercoronavirusstrainandlabelledwiththeimmunoperoxidaseantibody(IPA)techniqueforMAAdetectionshowedthatMAAconsistedmainlyofa
4、stronglylabelled,discontinuous,brush-likelayerofamorphousmaterial,strictlyassociatedwiththeinfectedcellmembranelBylightmicroscopy,reactivityofMAAwithhomologousandheterologousimmuneserumwassimilartothatofantigensdetectedbyIPAinethanol-fixedinfectedcells.IPAandIFAMA,b
5、utnothaemagglutination-inhibiting(Hi)andneutralizing(Nt)antibody,werestronglydecreasedbyabsorptionofimmuneserawithtrypsin-treatedglutaraldehyde-fixedcellculturesinfectedwithhomologousvirus.MAAIgGantibodiesweredetectedbyIFAMAinbothhumanandanimalsera.Serafrominfantssh
6、owinganHIandNt,butnotanIPA,antibodyresponsetoHCVOC43werealsofreeofdetectableiFAMAantibodytoHCVOC43.Humancoronavirus(HCV)OC43causesupperrespiratorytractsyndromesinhumans(Mclntoshetal.,1967;Kayeetal.,1971;Bradburne&Somerset,1972);neonatalcalfdiarrhoeacoronavirus(NCDCV
7、)isamajorcauseofdiarrhoeainnewborncalves(Stairetal.,1972;Mebusetal.,1973).Thesetwocoronavirusstrainshavebeenshowntopossesscloseantigenicrelationships(Gernaetal.,1981).Recently,Robb&Bond(1979)reportedtheexpressionofmousehepatitisvirusantigensinplasmamembraneofinfecte
8、dceils,buttheimmunologicalreactivityoftheseantigenswasnotinvestigated.Inthisreport,thepresenceofmembrane-associatedantigens(MAA)inHCVOC43-
