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Eitheroftheseapproachesmayfacilitatethepreparationoflentiviraldemonstratedbyimmunostainingwithavirus-specificMAb.Invectorsforlarge-scalepreclinicalstudiesand,ultimately,forclinicalcontrast,inoculationofthesecelllineswithfMHVintheabsenceofapplicationsinhuma
2、ns.bispecificscFvproteindidnotresultinanydetectableinfection.Inaddition,aderivativeofthemurinecelllineNIH-3T3stably454.IncorporationofModifiedVesicularexpressingtheEGFRcouldbeinfectedwithfMHV,whereasparentalNIH-3T3cellscouldnot,confirmingthespecificityoftheinfectionStomati
3、tisVirusG-Glycoprotein(VSV-G)intoviaEGFR.ThiswasfurthercorroboratedbythefindingthatinfectionInfectiousPseudotypedRetrovirusVectorsofhumantumorcelllinesusingthebispecificfusionproteincouldGhiabeH.Guibinga,1FrederickL.Hall,2ErlindaM.Gordon,2beblockedwithMAb23F8.1orMab425,the
4、anti-spikeandanti-ErkkiRuoslahti,3TheodoreFriedmann.1EGFRantibodiesrespectively,fromwhichthetwoscFvwere1Pediatrics,CenterforMolecularGenetics,Universityofderived.Inconclusion,fMHVcanbeselectivelytargetedtohumanCaliforniaSanDiego,LaJolla,CA;2EpeiusBiotechnologies,Lostumorce
5、llsbyusingabispecifictargetingdevice.ThisresultprovidesAngeles,CA;3BurnhamInstitute,LaJolla,CA.arationaleforfurtherinvestigationsontheuseofcoronavirusesasStudiesofcell-targetedgenedeliverybyVSV-G-pseudotypedanti-tumoragents.retroviruseshavebeenhamperedpartlybythelackofthre
6、e-dimensionalstructuralcharacterizationoftheVSV-Gprotein.456.DevelopmentofaHigh-TiterBCell-ModifiedvariantsofVSV-GcontainingpotentiallytargetingligandsSpecificSelf-InactivatingRetroviralVectorhavebeenfoundtointerferewithvectorproductionbydisruptionMelanieWerner,1JanineKrau
7、nus,2ChristopherBaum,2ThomasofnormalintracellulartraffickingmechanismsordefectivefusiogenicBrocker.1propertiesofthemodifiedVSV-Gprotein,withresultingineffective1InstituteforImmunology,Ludwig-Maximillians-University,assemblyintoinfectiousvirusparticles.Inthecurrentstudy,weM
8、unich,Germany;2ExperimentalCellTherapy,Departmentofdemonstrateproductionofinfectiousvirus
