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1、526_530.QXD8/22/20052:44PMPage526Coronavirus-associatedepizooticcatarrhalenteritisinferretsEXOTICSBruceH.Williams,DVM,DACVP;MattiKiupel,MS,Drmedvet;KeithH.West,DVM,PhD;SMALLANIMALS/JamesT.Raymond,DVM,DACVP;ChristopherK.Grant,DVM,PhD;LawrenceT.Glickman
2、,VMD,DrPHterizedashavinghighmorbidityandlowmortalitywithinitialsignsoflethargy,inappetence,andvomit-Objective—Tocharacterizeclinicalsignsandlesionsing.Subsequently,profusebrightgreendiarrheawithandidentifytheetiologicagentassociatedwithepi-highmucusco
3、ntentdeveloped,oftenaffecting100%ofzooticcatarrhalenteritisindomesticferrets.ferretsineachfacility.DiseaseseverityinindividualsDesign—Cross-sectionalstudy.washighlyvariable;olderferretshadmoresevereAnimals—119ferretswithepizooticdiarrheaofpre-signs,wh
4、ereasyoungferretshadmildorsubclinicalsumedviralcauseand5controlferrets.infection.Sincethattime,thisdiseasehasbeendiag-Procedure—ClinicalrecordsandbiopsyornecropsynosedthroughouttheUnitedStatesandinseveralspecimensofferretswithpresumedepizooticothercou
5、ntries,affectingferretsinbreedingandrescuecatarrhalenteritiswerereviewed.Immunohisto-facilitiesaswellaspetferrets,resultinginsubstantialchemicalstainingforcoronavirusantigenwasper-economiclossandemotionaldistress.Thediseasewasformedonparaffin-embedded
6、tissuesfromapproxi-namedepizooticcatarrhalenteritis(ECE)afterstudymately10%ofaffectedferretstoidentifyviralantigenattheArmedForcesInstituteofPathologyonthebasisanddetermineitsdistribution.Transmissionelectronofsimilaritiestoadiarrhealdiseaseofmink(epi
7、zooticmicroscopywasperformedonfecalsamplesandsec-1catarrhalgastroenteritis).tionsofjejunum.VirusisolationstudiesaswellasimmunofluorescenttestsforothersimilarvirusesInECEofferrets,asinepizooticcatarrhalgas-wereperformed.troenteritisofmink,coronavirus-l
8、ikeparticleswereseeninfeces,andacombinationofatrophicandResults—Characteristicmicroscopiclesionsconsis-tentwithintestinalcoronavirusinfection(vacuolarinflammatoryentericlesionscharacteristicofchronicdegenerationandnecrosisofvillusenterocytes;v
